Invitrogen

TLR2 Chimeric Recombinant Rabbit Monoclonal Antibody (TL2.1)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-48249

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Inhibition Assays (IA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

TL2.1

Immunogen

This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074691

Product Specific Information

Specificity: This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

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Gene Aliases: CD282; TIL4; TLR2

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UniProt ID: (Human) O60603

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Entrez Gene ID: (Human) 7097

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity
Process(es)
cytokine secretion involved in immune response MyD88-dependent toll-like receptor signaling pathway apoptotic process inflammatory response immune response signal transduction I-kappaB phosphorylation positive regulation of gene expression positive regulation of Wnt signaling pathway lipopolysaccharide-mediated signaling pathway interleukin-10 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide innate immune response positive regulation of transcription from RNA polymerase II promoter regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide
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