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Product Details
MA1-25649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
40C1285.6
Immunogen
A synthetic peptide corresponding to amino acids 55-70 (VLNLTHNQLRRLPAAN) of human TLR3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_795573
Product Specific Information
MA1-25649 detects TLR3 from human samples.
Target Information
TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD283; TLR 3; Toll-like receptor 3
Gene Aliases: AI957183; CD283; IIAE2; TLR3
UniProt ID: (Human) O15455, (Mouse) Q99MB1
Entrez Gene ID: (Dog) 482905, (Human) 7098, (Mouse) 142980
microglial cell activation
positive regulation of cytokine production
positive regulation of protein phosphorylation
toll-like receptor signaling pathway
microglial cell activation involved in immune response
immune system process
regulation of dendritic cell cytokine production
MyD88-independent toll-like receptor signaling pathway
defense response
inflammatory response
signal transduction
I-kappaB phosphorylation
response to virus
positive regulation of type I interferon production
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 3 signaling pathway
positive regulation of type III interferon production
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
response to dsRNA
positive regulation of interferon-gamma biosynthetic process
positive regulation of chemokine biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
defense response to virus
cellular response to exogenous dsRNA
extrinsic apoptotic signaling pathway
necroptotic signaling pathway
hyperosmotic response
I-kappaB kinase/NF-kappaB signaling
activation of NF-kappaB-inducing kinase activity
male gonad development
detection of virus
positive regulation of gene expression
negative regulation of MyD88-independent toll-like receptor signaling pathway
cellular response to interferon-beta
TRIF-dependent toll-like receptor signaling pathway
cellular response to drug
defense response to bacterium
positive regulation of apoptotic process
negative regulation of osteoclast differentiation
necroptotic process
cellular response to mechanical stimulus
cellular response to interferon-gamma
apoptotic signaling pathway
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