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TLR7 Antibody (PA1-28109) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TLR7 in Ramos cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and TLR7 Polyclonal Antibody (Product # PA1-28109) (Green) at a dilution of 1:50 was used as a primary antibody. Red : Tubulin. Blue: DAPI.
Product Details
PA1-28109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1961065
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
This antibody will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.
Target Information
TLR7 is a 10 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. TLR7 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). TLR7 is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. TLR7 also acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. TLR7 recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: RP23-139P21.3; Toll-like receptor 7; toll-like receptor 7-like
Gene Aliases: RGD1563357; TLR7; TLR7-like; UNQ248/PRO285
UniProt ID: (Human) Q9NYK1, (Mouse) P58681
Entrez Gene ID: (Human) 51284, (Mouse) 170743, (Rat) 317468
microglial cell activation
regulation of protein phosphorylation
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
I-kappaB phosphorylation
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
toll-like receptor 7 signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of inflammatory response
defense response to virus
cellular response to mechanical stimulus
immune system process
immune response
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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