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Invitrogen
TLR7 Recombinant Rabbit Monoclonal Antibody (JA93-11)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
TLR7 Antibody (MA5-32658) in WB
Western blot was performed using TLR7 Recombinant Rabbit Monoclonal Antibody (JA93-11) (Product # MA5-32658) and a 120 kDa band corresponding to TLR7 was observed across tested panel. Whole cell extracts (40 µg lysate) of Daudi (Lane 1), T-47D (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), Hep G2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bl... View More
Product Details
MA5-32658
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA93-11
Immunogen
Synthetic peptide within Human TLR7 aa 1000-1049
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809935
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
The immunogen for MA5-32658 is Human TLR7 C-terminal aa 1036-1049 (DNHVAYSQVF KETV).
Target Information
TLR7 is a 10 amino acid long highly conserved membrane protein belonging to the Toll-like receptor (TLR) family with 27 LRR (leucine-rich) repeats and a TIR domain. TLR7 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 is activated by infections with single-stranded RNA viruses, including influenza virus and vesicular stomatitis virus (VSV). TLR7 is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8. TLR7 also acts via MyD88 and TRAF6, leading to NF-kappaB activation, cytokine secretion and the inflammatory response. TLR7 recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RP23-139P21.3; Toll-like receptor 7; toll-like receptor 7-like
Gene Aliases: TLR7; TLR7-like; UNQ248/PRO285
UniProt ID: (Human) Q9NYK1
Entrez Gene ID: (Human) 51284
microglial cell activation
regulation of protein phosphorylation
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
I-kappaB phosphorylation
positive regulation of chemokine production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
toll-like receptor 7 signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of inflammatory response
defense response to virus
cellular response to mechanical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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