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Invitrogen
TLR8 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
TLR8 Antibody (PA5-80137) in WB
Western Blot was performed using Anti-TLR8 Polyclonal Antibody (Product # PA5-80137) and a 150 kDa band corresponding to full length Toll-like receptor 8 was observed in ssRNA40 stimulated THP-1 cells but not in B-cells, which are reported negative for TLR8. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), IFN-gamma stimulated THP-1 (Lane 2), IFN-gamma stimulated THP-1 cells treated with ssRNA40 (Lane 3), Ramos (Lane 4), and Raji (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved pro... View More
Product Details
PA5-80137
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat TLR8 (1013-1029aa ENDSRYDNLYIDSIRQY).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747251
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The TLR8 protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The TLR8 gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen 288; CD288; Toll-like receptor 8
Gene Aliases: CD288; TLR8; UNQ249/PRO286
UniProt ID: (Human) Q9NR97, (Mouse) P58682
Entrez Gene ID: (Human) 51311, (Mouse) 170744, (Rat) 684440
microglial cell activation
regulation of protein phosphorylation
toll-like receptor signaling pathway
immune system process
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
signal transduction
I-kappaB kinase/NF-kappaB signaling
response to virus
toll-like receptor 8 signaling pathway
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of interleukin-8 biosynthetic process
regulation of cytokine secretion
defense response to virus
immunoglobulin mediated immune response
toll-like receptor 9 signaling pathway
cellular response to mechanical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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