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TMEM119 Antibody (PA5-119617) in IHC (P)
TMEM119 Polyclonal Antibody detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TMEM119 stained by TMEM119 Polyclonal Antibody (Product # PA5-119617) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-119617
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse TMEM119. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913190
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OBIF; Osteoblast induction factor; Transmembrane protein 119
Gene Aliases: AW208946; BC025600; obif; Tmem119
UniProt ID: (Mouse) Q8R138
Entrez Gene ID: (Mouse) 231633
Performance Guarantee
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