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TMEM119 Antibody (PA5-120705) in WB
Western blot analysis of TMEM119 in extracts of various cell lines. Samples were incubated with TMEM119 Polyclonal antibody (Product # PA5-120705) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-120705
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 160-270 of human TMEM119 (NP_859075.2).
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914277
Product Specific Information
Positive test controls include: Mouse liver, Mouse brain, Rat brain.
Immunogen sequence: SRPEEALDSS RQLQADILAA TQNLKSPTRA ALGGGDGARM VEGRGAEEEE KGSQEGDQEV QGHGVPVETP EAQEEPCSGV LEGAVVAGEG QGELEGSLLL AQEAQGPVGP P
Target Information
Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OBIF; Osteoblast induction factor; Transmembrane protein 119; transmembrane protein 119 homolog
Gene Aliases: AW208946; BC025600; obif; RGD1307573; Tmem119
UniProt ID: (Mouse) Q8R138
Entrez Gene ID: (Mouse) 231633, (Rat) 304581
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