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TMEM119 Monoclonal Antibody (1E7A2)

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TMEM119 Antibody (66948-1-IG) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 66948-1-IG) (TMEM119 antibody) at dilution of 1:5,0000 incubated at room temperature for 1.5 hours.

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Product Details

66948-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC/IF)

View 11 publications 11 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rabbit, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

1E7A2

Immunogen

TMEM119 Fusion Protein Ag26286

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fibrinogen primes the microglial NLRP3 inflammasome and propagates pro-inflammatory signaling via extracellular vesicles: Implications for blood-brain barrier dysfunction.

Neurobiology of disease

Roseborough AD,Zhu Y,Zhao L,Laviolette SR,Pasternak SH,Whitehead SN

Wed Feb 01 00:00:00 EST 2023

Plasma derived extracellular vesicle biomarkers of microglia activation in an experimental stroke model.

Journal of neuroinflammation

Roseborough AD,Myers SJ,Khazaee R,Zhu Y,Zhao L,Iorio E,Elahi FM,Pasternak SH,Whitehead SN

Tue Jan 31 00:00:00 EST 2023

HC067047 Ameliorates Sepsis-associated Encephalopathy by Suppressing Endoplasmic Reticulum Stress and Oxidative Stress-Induced Pyroptosis in the Hippocampi of Mice.

Neuroscience

Zhong X,Wang Y,Liu D,Liang Y,Liu W,Huang Y,Xie L,Cao W,Xu Y,Chen L

Mon May 01 00:00:00 EDT 2023

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation.

Autophagy

Cai W,Hu M,Li C,Wu R,Lu D,Xie C,Zhang W,Li T,Shen S,Huang H,Qiu W,Liu Q,Lu Y,Lu Z

Sat Apr 01 00:00:00 EDT 2023

Differential Effects of APOE Genotype on MicroRNA Cargo of Cerebrospinal Fluid Extracellular Vesicles in Females With Alzheimer's Disease Compared to Males.

Frontiers in cell and developmental biology

Sandau US,McFarland TJ,Smith SJ,Galasko DR,Quinn JF,Saugstad JA

Thu Aug 17 00:00:00 EDT 2023

QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.

Molecular immunology

Deng L,Wang W,Bian P,Wu M,Wang L,Lei Y,Lu Z,Zhai D

Mon Aug 01 00:00:00 EDT 2022

Erxian decoction inhibits apoptosis by activating Akt1 and repairs spinal cord injury in rats.

Heliyon

Li E,Yan R,Yan K,Huang R,Zhang R,Wen Y,Li S,Li P,Ma Q,Liao B

Tue Nov 01 00:00:00 EDT 2022

Tumor Microenvironment and Immune Escape in the Time Course of Glioblastoma.

Molecular neurobiology

Virtuoso A,De Luca C,Cirillo G,Riva M,Romano G,Bentivegna A,Lavitrano M,Papa M,Giovannoni R

Tue Nov 01 00:00:00 EDT 2022

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Journal of neuroinflammation

Liu Q,Zhou S,Wang X,Gu C,Guo Q,Li X,Zhang C,Zhang N,Zhang L,Huang F

Mon Jun 20 00:00:00 EDT 2022

Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia.

ACS nano

Shen S,Cheng X,Zhou L,Zhao Y,Wang H,Zhang J,Sun X,Wang Y,Shu Y,Xu Y,Tao Y,Li M,Lu Z,Cai W,Nie G,Qiu W

Tue Nov 22 00:00:00 EST 2022

An increase in Semaphorin 3A biases the axonal direction and induces an aberrant dendritic arborization in an in vitro model of human neural progenitor differentiation.

Cell & bioscience

Ferretti G,Romano A,Sirabella R,Serafini S,Maier TJ,Matrone C

Tue Nov 08 00:00:00 EST 2022

Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.

Frontiers in oncology

Jia M,Zhang W,Zhu J,Huang C,Zhou J,Lian J,Wang Y,Teng H,Huang Z

Tue Aug 24 00:00:00 EDT 2021

Flow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat model.

Scientific reports

Lin J,Zhou L,Luo Z,Adam MI,Zhao L,Wang F,Luo D

Tue Dec 07 00:00:00 EST 2021

Can quantifying morphology and TMEM119 expression distinguish between microglia and infiltrating macrophages after ischemic stroke and reperfusion in male and female mice?

Journal of neuroinflammation

Young KF,Gardner R,Sariana V,Whitman SA,Bartlett MJ,Falk T,Morrison HW

Mon Feb 22 00:00:00 EST 2021

TMEM119 facilitates ovarian cancer cell proliferation, invasion, and migration via the PDGFRB/PI3K/AKT signaling pathway.

Journal of translational medicine

Sun T,Bi F,Liu Z,Yang Q

Wed Mar 17 00:00:00 EDT 2021

TMEM119 facilitates ovarian cancer cell proliferation, invasion, and migration via the PDGFRB/PI3K/AKT signaling pathway.

Journal of translational medicine

Sun T,Bi F,Liu Z,Yang Q

Wed Mar 17 00:00:00 EDT 2021

Age and sex differences in primary microglia culture: A comparative study.

Journal of neuroscience methods

Ngwa C,Qi S,Mamun AA,Xu Y,Sharmeen R,Liu F

Wed Dec 01 00:00:00 EST 2021

Contribution of the P2X4 Receptor in Rat Hippocampus to the Comorbidity of Chronic Pain and Depression.

ACS chemical neuroscience

Li L,Zou Y,Liu B,Yang R,Yang J,Sun M,Li Z,Xu X,Li G,Liu S,Greffrath W,Treede RD,Li G,Liang S

Wed Dec 16 00:00:00 EST 2020

Methane-Rich Saline Alleviates CA/CPR Brain Injury by Inhibiting Oxidative Stress, Microglial Activation-Induced Inflammatory Responses, and ER Stress-Mediated Apoptosis.

Oxidative medicine and cellular longevity

Cui R,Liu S,Wang C,Liu T,Ren J,Jia Y,Tong Y,Liu C,Zhang J

Thu May 13 00:00:00 EDT 2021

Electroacupuncture Treatment Attenuates Paclitaxel-Induced Neuropathic Pain in Rats via Inhibiting Spinal Glia and the TLR4/NF-κB Pathway.

Journal of pain research

Zhao YX,Yao MJ,Liu Q,Xin JJ,Gao JH,Yu XC

Wed Apr 13 00:00:00 EDT 2022

Characterization of brain-derived extracellular vesicles reveals changes in cellular origin after stroke and enrichment of the prion protein with a potential role in cellular uptake.

Journal of extracellular vesicles

Brenna S,Altmeppen HC,Mohammadi B,Rissiek B,Schlink F,Ludewig P,Krisp C,Schlüter H,Failla AV,Schneider C,Glatzel M,Puig B,Magnus T

Thu Aug 27 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: OBIF; Osteoblast induction factor; Transmembrane protein 119; transmembrane protein 119 homolog

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Gene Aliases: AW208946; BC025600; OBIF; PSEC0199; RGD1307573; TMEM119; UNQ731/PRO1415

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UniProt ID: (Human) Q4V9L6, (Mouse) Q8R138

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Entrez Gene ID: (Human) 338773, (Rabbit) 100354483, (Pig) 100621401, (Mouse) 231633, (Rat) 304581

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Function(s)

molecular_function protein binding

Process(es)

osteoblast differentiation spermatogenesis biological_process positive regulation of bone mineralization biomineral tissue development positive regulation of osteoblast proliferation positive regulation of osteoblast differentiation spermatid differentiation positive regulation of bone development ossification cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TMEM119 Monoclonal Antibody (1E7A2)

TMEM119 Antibody (66948-1-IG) in WB

Product Details

66948-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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