Invitrogen

TMPRSS2 Polyclonal Antibody

TMPRSS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TMPRSS2 Antibody (PA5-14264) in IHC (P)

Immunohistochemistry analysis of TMPRSS2 in formalin fixed and paraffin embedded human testis tissue. Samples were incubated with TMPRSS2 polyclonal antibody (Product # PA5-14264) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

TMPRSS2 Antibody in Flow Cytometry (Flow)

TMPRSS2 Antibody in Immunocytochemistry (ICC/IF)

TMPRSS2 Antibody in Western Blot, Flow Cytometry (WB, Flow)

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Product Details

PA5-14264

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

1:10-1:50

View 4 publications 4 publications

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TMPRSS2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2256213

Target Information

TMPRSS2 is a protein that belongs to the serine protease family. The protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. Its gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cells.

Frontiers in neuroscience

McQuaid C,Solorzano A,Dickerson I,Deane R

PA5-14264 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry to suggest that in addition to ACE2, gangliosides are also an important entry point of SARS-CoV-2/SP into these cells.

Tue Mar 07 00:00:00 EST 2023

Highly restricted SARS-CoV-2 receptor expression and resistance to infection by primary human monocytes and monocyte-derived macrophages.

Journal of leukocyte biology

Zankharia U,Yadav A,Yi Y,Hahn BH,Collman RG

Published figure using TMPRSS2 polyclonal antibody (Product # PA5-14264) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Peptidomimetic inhibitors of TMPRSS2 block SARS-CoV-2 infection in cell culture.

Communications biology

Wettstein L,Knaff PM,Kersten C,Müller P,Weil T,Conzelmann C,Müller JA,Brückner M,Hoffmann M,Pöhlmann S,Schirmeister T,Landfester K,Münch J,Mailänder V

PA5-14264 was used in Flow cytometry/Cell sorting to report antivirally active and stable TMPRSS2 inhibitors with prospects for further preclinical and clinical development as antiviral agents against SARS-CoV-2 and other TMPRSS2-dependent viruses.

Fri Jul 08 00:00:00 EDT 2022

Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 Patients.

Stem cell reports

Huerga Encabo H,Grey W,Garcia-Albornoz M,Wood H,Ulferts R,Aramburu IV,Kulasekararaj AG,Mufti G,Papayannopoulos V,Beale R,Bonnet D

PA5-14264 was used in Immunocytochemistry to study hematopoietic stem/progenitor cells and different erythroid progenitor populations to assess if they can be infected by SARS-CoV-2.

Tue Mar 09 00:00:00 EST 2021

Human Erythroid Progenitors Are Directly Infected by SARS-CoV-2: Implications for Emerging Erythropoiesis in Severe COVID-19 Patients.

Stem cell reports

Huerga Encabo H,Grey W,Garcia-Albornoz M,Wood H,Ulferts R,Aramburu IV,Kulasekararaj AG,Mufti G,Papayannopoulos V,Beale R,Bonnet D

PA5-14264 was used in Immunocytochemistry to study hematopoietic stem/progenitor cells and different erythroid progenitor populations to assess if they can be infected by SARS-CoV-2.

Tue Mar 09 00:00:00 EST 2021

Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection.

Journal of neuroinflammation

Torices S,Cabrera R,Stangis M,Naranjo O,Fattakhov N,Teglas T,Adesse D,Toborek M

Published figure using TMPRSS2 polyclonal antibody (Product # PA5-14264) in Western Blot

Thu Jul 29 00:00:00 EDT 2021

Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection.

Journal of neuroinflammation

Torices S,Cabrera R,Stangis M,Naranjo O,Fattakhov N,Teglas T,Adesse D,Toborek M

Published figure using TMPRSS2 polyclonal antibody (Product # PA5-14264) in Western Blot

Thu Jul 29 00:00:00 EDT 2021

Antiviral Potential of the Antimicrobial Drug Atovaquone against SARS-CoV-2 and Emerging Variants of Concern.

ACS infectious diseases

Carter-Timofte ME,Arulanandam R,Kurmasheva N,Fu K,Laroche G,Taha Z,van der Horst D,Cassin L,van der Sluis RM,Palermo E,Di Carlo D,Jacobs D,Maznyi G,Azad T,Singaravelu R,Ren F,Hansen AL,Idorn M,Holm CK,Jakobsen MR,van Grevenynghe J,Hiscott J,Paludan SR,Bell JC,Seguin J,Sabourin LA,Côté M,Diallo JS,Alain T,Olagnier D

Published figure using TMPRSS2 polyclonal antibody (Product # PA5-14264) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Antiviral Potential of the Antimicrobial Drug Atovaquone against SARS-CoV-2 and Emerging Variants of Concern.

ACS infectious diseases

Published figure using TMPRSS2 polyclonal antibody (Product # PA5-14264) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entry.

Nature communications

Chen Y,Lear TB,Evankovich JW,Larsen MB,Lin B,Alfaras I,Kennerdell JR,Salminen L,Camarco DP,Lockwood KC,Tuncer F,Liu J,Myerburg MM,McDyer JF,Liu Y,Finkel T,Chen BB

PA5-14264 was used in Proximity Ligation Assay (PLA) to identify small molecules that reduce surface expression of TMPRSS2.

Wed Jun 23 00:00:00 EDT 2021

Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.

Nature communications

Wettstein L,Weil T,Conzelmann C,Müller JA,Groß R,Hirschenberger M,Seidel A,Klute S,Zech F,Prelli Bozzo C,Preising N,Fois G,Lochbaum R,Knaff PM,Mailänder V,Ständker L,Thal DR,Schumann C,Stenger S,Kleger A,Lochnit G,Mayer B,Ruiz-Blanco YB,Hoffmann M,Sparrer KMJ,Pöhlmann S,Sanchez-Garcia E,Kirchhoff F,Frick M,Münch J

PA5-14264 was used in Surface Plasmon Resonance to identify factors of the respiratory tract that block SARS-CoV-2 infection, revealing the presence of α1-antitrypsin, a highly abundant circulating serine protease inhibitor.

Fri Mar 19 00:00:00 EDT 2021

Infection of bat and human intestinal organoids by SARS-CoV-2.

Nature medicine

Zhou J,Li C,Liu X,Chiu MC,Zhao X,Wang D,Wei Y,Lee A,Zhang AJ,Chu H,Cai JP,Yip CC,Chan IH,Wong KK,Tsang OT,Chan KH,Chan JF,To KK,Chen H,Yuen KY

Wed Jul 01 00:00:00 EDT 2020

Development and validation of IMMUNO-COV™: a high-throughput clinical assay for detecting antibodies that neutralize SARS-CoV-2.

bioRxiv : the preprint server for biology

Vandergaast R,Carey T,Reiter S,Lech P,Gnanadurai C,Tesfay M,Buehler J,Suksanpaisan L,Naik S,Brunton B,Recker J,Haselton M,Ziegler C,Roesler A,Mills JR,Theel E,Weaver SC,Rafael G,Roforth MM,Jerde C,Tran S,Diaz RM,Bexon A,Baum A,Kyratsous CA,Peng KW,Russell SJ

PA5-14264 was used in Flow cytometry/Cell sorting to demonstrate that the IMMUNO-COV™ assay accurately quantitates SARS-CoV-2 neutralizing antibodies in human sera and therefore is a potentially valuable addition to the currently available serological tests.

Wed May 27 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: EC 3.4.219; epitheliasin; FLJ41954; MGC6821; Serine protease 10; TMPS2; Transmembrane protease serine 2

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Gene Aliases: PP9284; PRSS10; TMPRSS2

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UniProt ID: (Human) O15393

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Entrez Gene ID: (Human) 7113

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Function(s)

serine-type endopeptidase activity scavenger receptor activity protein binding serine-type peptidase activity serine protease

Process(es)

proteolysis receptor-mediated endocytosis protein autoprocessing positive regulation of viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TMPRSS2 Polyclonal Antibody

TMPRSS2 Antibody (PA5-14264) in IHC (P)

Product Details

PA5-14264

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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