Invitrogen

TNF alpha Monoclonal Antibody (4F4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

10 References

View all (210) TNF alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TNF alpha Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 3

TNF alpha Antibody (MP390) in IHC

Journal of inflammation (London, England) 2010 - Figure 7 Immunohistochemical detection of TNF-alpha in biopsies of DNCB-induced rashes treated with various nanocrystalline silver-derived solutions . (A) Representative images are shown for immunohistochemical detection of TNF-alpha after 72 h treatment of negative controls with distilled water (i), and DNCB-induced porcine contact dermatitis rashes with distilled water (positive controls) (ii), or nanocry... View More

TNF alpha Antibody in Flow Cytometry (Flow)

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Product Details

MP390

Applications

Tested Dilution

Publications

Western Blot (WB)

0.02 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

0.1-0.25 µg/mL (coating)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Pig

Published species

Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4F4

Immunogen

Recombinant porcine TNF alpha (Baculovirus-derived)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223611

Product Specific Information

MP390 detects natural and recombinant TNF alpha, with a predicted molecular weight of approximately 25 kDa. This antibody will not cross-react with human or mouse TNFa, mouse TNFb, or porcine TNFb.

This is a carrier-free low endotoxin formulation, <1.0 EU/mg.

This product can be used as a coating antibody in a sandwich ELISA (100 µg/well) in combination with biotin-labeled TNFa monoclonal antibody (Product # M302B, 0.1-0.65 µg/mL). For Western blot, this antibody has been successfully used in conjunction with 0.02 µg/mL Biotin-conjugated Goat-anti-Mouse IgG (Product # 31800), 0.05 µg/mL HRP-conjugated Streptavidin (Product # N100), and recombinant Porcine TNFa (Product # RPTNFAI), followed by visualization using SuperSignal® West Pico Chemiluminescent Substrate (Product # 34080).

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma.

Veterinary immunology and immunopathology

Wyns H,Croubels S,Demeyere K,Watteyn A,De Backer P,Meyer E

MP390 was used in ELISA to develop a cytometric bead array to detect porcine cytokines and acute phase proteins

Tue Jan 15 00:00:00 EST 2013

Does nanocrystalline silver have a transferable effect?

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Nadworny PL,Landry BK,Wang J,Tredget EE,Burrell RE

MP390 was used in immunohistochemistry to study the antiinflammatory effect of nanocrystalline silver

Tue Sep 14 00:00:00 EDT 2010

Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft.

Annals of plastic surgery

Cayci C,Wahlquist TC,Seckin SI,Ozcan V,Tekinay AB,Martens TP,Oz MC,Ascherman JA

MP390 was used in immunohistochemistry to study the role of Naringenin in development of neointima following arterial reconstruction

Fri Jan 01 00:00:00 EST 2010

Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.

Journal of inflammation (London, England)

Nadworny PL,Wang J,Tredget EE,Burrell RE

MP390 was used in immunohistochemistry to evaulate the benefit of nanocrystalline silver in porcine contact dermatitis

Fri Feb 19 00:00:00 EST 2010

Production of tumor necrosis factor-alpha from porcine nucleus pulposus cells at various time points in cell culture under conditions of nutritional deficiency.

Cytokine

Murata Y,Olmarker K,Larsson K,Takahashi K,Rydevik B

MP390 was used in immunohistochemistry to investigate the changes of tumor necrosis factor alpha expression in pigs under starvation conditions

Mon May 01 00:00:00 EDT 2006

Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production.

Viral immunology

Charerntantanakul W,Platt R,Roth JA

MP390 was used in flow cytometry to show that porcine reproductive and respiratory syndrome virus can suppress T cells

Wed Feb 28 00:00:00 EST 2007

Lipopolysaccharide-induced impairment of classical swine fever virus infection in monocytic cells is sensitive to 2-aminopurine.

Antiviral research

Knoetig SM,McCullough KC,Summerfield A

MP390 was used in ELISA to study the effect of lipopolysaccharide and 2-aminopurine on classical swine fever virus infection

Tue Jan 01 00:00:00 EST 2002

Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.

Clinical and diagnostic laboratory immunology

Aasted B,Bach P,Nielsen J,Lind P

Published figure using TNF alpha monoclonal antibody (Product # MP390) in Flow Cytometry

Fri Nov 01 00:00:00 EST 2002

Detection of monokines in paraffin-embedded tissues of pigs using polyclonal antibodies.

Veterinary research

Salguero FJ,Mekonnen T,Ruiz-Villamor E,Sánchez-Cordón PJ,Gómez-Villamandos JC

MP390 was used in ELISA to investigate the reliability of antibodies for the measurement of monokines in paraffin-embedded pig tissues

Mon Jan 28 00:00:00 EST 2002

Cellular changes and cytokine expression in the ilea of gnotobiotic piglets resulting from peroral Salmonella typhimurium challenge.

Infection and immunity

Trebichavský I,Dlabac V,Reháková Z,Zahradnícková M,Splíchal I

MP390 was used in immunocytochemistry to investigate the changes of cytokine gene expression in piglets infected with Salmonella typhimurium

Mon Dec 01 00:00:00 EST 1997

Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha precursor; Tumor necrosis factor ligand superfamily member 2

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Gene Aliases: TNF; TNFA; TNFSF2

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UniProt ID: (Pig) P23563

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Entrez Gene ID: (Pig) 397086

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Function(s)

protease binding identical protein binding

Process(es)

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TNF alpha Monoclonal Antibody (4F4)

TNF alpha Antibody (MP390) in IHC

Product Details

MP390

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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