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TNF beta Antibody (500-P31BBT-25UG) in ELISA
Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of TNF beta Polyclonal Antibody, Biotin (Product # 500-P31BBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech TNF beta Polyclonal Antibody (Product # 500-P31B-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-beta.
Product Details
500-P31BBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 18.6 kDa Recombinant Human TNF-beta
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930387
Product Specific Information
AA Sequence of recombinant protein: MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-beta. Anti-Human TNF-beta-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TNF-beta (500-P31B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TNF-beta.
Western Blot: To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P31BBT-1MG will be provided as 2 x 500 µg
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAMA-25N12.13; LT-alpha; Lymphotoxin-alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF β; TNF-beta; TNFbeta; TNFβ; tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1
Gene Aliases: LT; LTA; TNFB; TNFSF1; TNLG1E
UniProt ID: (Human) P01374
Entrez Gene ID: (Human) 4049
response to hypoxia
positive regulation of chronic inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
apoptotic process
humoral immune response
signal transduction
cell-cell signaling
response to nutrient
response to lipopolysaccharide
positive regulation of interferon-gamma production
tumor necrosis factor-mediated signaling pathway
response to drug
positive regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of fibroblast proliferation
lymph node development
defense response to Gram-positive bacterium
positive regulation of glial cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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