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Invitrogen
TNF beta Monoclonal Antibody (359-81-11), APC, eBioscience™
FIGURE: 1 / 1
TNF beta Antibody (17-7327-81) in Flow
CD4-enriched human peripheral blood cells were polarized under Th1 conditions (with Human IL-12 Recombinant Protein (Product # 34-8129-82) and Human IL-2 Recombinant Protein (Product # BMS334) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product #00-4980-03) (left) or with Cell Stimulation Cocktail plus Protein Transport Inhibitors (Product #00-4975-03) (right) for 5 hours. Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and pr... View More
Product Details
17-7327-81
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
359-81-11
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469509
Product Specific Information
Description: The 359-81-11 antibody reacts with Tumor Necrosis Factor-beta (TNF-beta), a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and other target cells.
Applications Reported:The 359-81-11 antibody has been reported for use in detection of human TNF beta by ELISA and intracellular staining for flow cytometric analysis.
Has been tested by intracellular staining and flow cytometric analysis of TH1 polarized restimulated normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAMA-25N12.13; LT-alpha; Lymphotoxin-alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF β; TNF-beta; TNFbeta; TNFβ; tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1
Gene Aliases: LT; LTA; TNFB; TNFSF1; TNLG1E
UniProt ID: (Human) P01374
Entrez Gene ID: (Human) 4049
response to hypoxia
positive regulation of chronic inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
apoptotic process
humoral immune response
signal transduction
cell-cell signaling
response to nutrient
response to lipopolysaccharide
positive regulation of interferon-gamma production
tumor necrosis factor-mediated signaling pathway
response to drug
positive regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of fibroblast proliferation
lymph node development
defense response to Gram-positive bacterium
positive regulation of glial cell proliferation
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