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TNFAIP6 Antibody (PA5-47253) in ICC/IF
Immunocytochemistry analysis of TNFAIP6 in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in TNFAIP6 polyclonal antibody (Product # PA5-47253) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSG-6 Trp18-Leu277
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608216
Product Specific Information
In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant mouse TSG-6 is observed and less than 2% cross-reactivity with recombinant human TSG-14 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The TSG6 gene is transcribed in normal fibroblasts and activated by binding of the cytokines TNF alpha and IL-1 at AP-1 and NF-IL6 sites in its promoter. TSG-6 is a glycoprotein and a member of the hyaluronan-binding protein family, which includes cartilage link protein, proteoglycan core protein and the adhesion receptor CD44. TSG-6 is highly homologous to CD44, particularly in the hyaluronic acid-binding domain. TSG-6 is found in TNF-treated cells; its expression is rapidly activated by TNF alpha, IL-1 and lipopolysaccharide in normal fibroblasts, peripheral blood mononuclear cells, synovial cells and chondrocytes. The presence of TSG-6 in synovial fluid suggests a possible role in rheumatoid arthritis. TSG-6 forms a stable complex with components of the serine protease inhibitor, inter-alpha-inhibitor (I alpha I)). TSG-6 potentiates the inhibitory effect of l alpha l on the protease activity of plasmin. Through their cooperative inhibitory effect on plasmin, TSG-6 and l alpha l can modulate the protease network and thus inhibit inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hyaluronate-binding protein; TNF alpha-induced protein 6; TNF-stimulated gene 6 protein; TSG-6; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor, alpha induced protein 6; tumor necrosis factor, alpha-induced protein 6; Tumor necrosis factor-inducible gene 6 protein; tumor necrosis factor-stimulated gene-6 protein
Gene Aliases: TNFAIP6; TSG-6; TSG6
UniProt ID: (Human) P98066
Entrez Gene ID: (Human) 7130
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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