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TNFR1 Antibody (PA5-85174) in WB
Western blot analysis of TNFR1 in A549 whole cell and membrane extracts using TNFR1 polyclonal antibody (Product # PA5-85174) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
Product Details
PA5-85174
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TNF Receptor I.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792320
Product Specific Information
Keep as concentrated solution.
Positive Control: A549, A549 membrane fraction extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD120a; CD120a antigen; MGC19588; n-smase activation assoc. factor; p55; p60; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF-R1; TNF-RI; TNFR; TNFR I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; Tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Gene Aliases: CD120a; FPF; MS5; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR1-d2; TNFR55; TNFR60; TNFRSF1A
UniProt ID: (Human) P19438
Entrez Gene ID: (Human) 7132
prostaglandin metabolic process
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of tumor necrosis factor-mediated signaling pathway
viral process
cytokine-mediated signaling pathway
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of inflammatory response
cellular response to mechanical stimulus
death-inducing signaling complex assembly
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
positive regulation of ceramide biosynthetic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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