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TNFR1 (soluble) Antibody (500-P143BT-25UG) in WB
Western Blot: To detect hsTNF-Receptor I by Western Blot analysis TNFR1 (soluble) Polyclonal Antibody, Biotin (Product # 500-P143BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P143BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 18.3 kDa Recombinant Human TNF Receptor Type I
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929469
Product Specific Information
AA Sequence of recombinant protein: MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type I.
Western Blot: To detect hsTNF-Receptor I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hsTNF-Receptor I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P143BT-1MG will be provided as 2 x 500 µg
Target Information
TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD120a; CD120a antigen; MGC19588; n-smase activation assoc. factor; p55; p60; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF-R1; TNF-RI; TNFR; TNFR I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; Tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Gene Aliases: CD120a; FPF; MS5; p55; p55-R; p60; TBP1; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR1-d2; TNFR55; TNFR60; TNFRSF1A
UniProt ID: (Human) P19438
Entrez Gene ID: (Human) 7132
prostaglandin metabolic process
inflammatory response
immune response
I-kappaB kinase/NF-kappaB signaling
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
regulation of tumor necrosis factor-mediated signaling pathway
viral process
cytokine-mediated signaling pathway
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of inflammatory response
cellular response to mechanical stimulus
death-inducing signaling complex assembly
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
positive regulation of ceramide biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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