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TNFR2 (soluble) Antibody (500-P168-1MG) in WB
Western Blot: To detect Human sTNF Receptor Type II by Western Blot analysis TNFR2 (soluble) Polyclonal Antibody (Product # 500-P168-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P168-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human sTNF Receptor Type II
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929601
Product Specific Information
AA Sequence of recombinant protein: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type II matrix.
Sandwich ELISA: To detect Human sTNF Receptor Type II by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type II (500-P168Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTNF Receptor Type II.
Western Blot: To detect Human sTNF Receptor Type II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTNF Receptor Type II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD120b; p75; p75 TNF receptor; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF-R2; TNF-RII; TNFR; TNFR II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
Gene Aliases: CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; TNFRSF1B
UniProt ID: (Human) P20333
Entrez Gene ID: (Human) 7133
inflammatory response
immune response
multicellular organism development
aging
intrinsic apoptotic signaling pathway in response to DNA damage
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
regulation of apoptotic process
positive regulation of MAPK cascade
negative regulation of inflammatory response
RNA destabilization
positive regulation of membrane protein ectodomain proteolysis
cellular response to lipopolysaccharide
cellular response to growth factor stimulus
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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