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TRAIL (soluble) Antibody (500-P135BT-25UG) in WB
Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis TRAIL (soluble) Polyclonal Antibody, Biotin (Product # 500-P135BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P135BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 19.6 kDa Recombinant Human sTRAIL/Apo2L
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929424
Product Specific Information
AA Sequence of recombinant protein: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human sTRAIL/Apo2L by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTRAIL/Apo2L (500-P135) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sTRAIL/Apo2L.
Western Blot: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P135BT-1MG will be provided as 2 x 500 µg
Target Information
TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo-2 ligand; Apo-2L; CD253; CD253 antigen; chemokine tumor necrosis factor ligand superfamily member 10; Protein TRAIL; TNF superfamily member 10; TNF-related apoptosis inducing ligand TRAIL; TNF-related apoptosis-inducing ligand; TRAIL protein; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; Tumor necrosis factor ligand superfamily member 10
Gene Aliases: Apo-2L; APO2L; CD253; TL2; TNFSF10; TNLG6A; TRAIL
UniProt ID: (Human) P50591
Entrez Gene ID: (Human) 8743
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
immune response
signal transduction
cell surface receptor signaling pathway
cell-cell signaling
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of release of cytochrome c from mitochondria
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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