Proteintech

TRBP Polyclonal Antibody

TRBP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

TRBP Antibody (15753-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using TARBP2 antibody 15753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

TRBP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRBP Antibody in Immunoprecipitation (IP)

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Product Details

15753-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TRBP Fusion Protein Ag8255 (1-366 aa encoded by BC005860)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSEEEQGSG TTTGCGLPSI EQMLAANPGK TPISLLQEYG TRIGKTPVYD LLKAEGQAHQ PNFTFRVTVG DTSCTGQGPS KKAAKHKAAE VALKHLKGGS MLEPALEDSS SFSPLDSSLP EDIPVFTAAA AATPVPSVVL TRSPPMELQP PVSPQQSECN PVGALQELVV QKGWRLPEYT VTQESGPAHR KEFTMTCRVE RFIEIGSGTS KKLAKRNAAA KMLLRVHTVP LDARDGNEVE PDDDHFSIGV GSRLDGLRNR GPGCTWDSLR NSVGEKILSL RSCSLGSLGA LGPACCRVLS ELSEEQAFHV SYLDIEELSL SGLCQCLVEL STQPATVCHG SATTREAARG EAARRALQYL KIMAGSK (1-366 aa encoded by BC005860)

Target Information

TRBP belongs to the family of dsRNA-binding proteins. It consists of highly conserved 2 dsRNA-binding domains and C-terminal basic region. It is a unique cellular protein capable of binding to HIV-TAR RNA and stimulates expression of HIV-1 long terminal repeats. It also inhibits the host antiviral and anti-proliferative mechanisms by directly binding to IFN-regulated dsRNA-dependent protein kinase (PKR). TRBP associates with Ago2 protein and forms an integral component of Dicer-containing complex. It has a role in micro RNA processing and in RISC assembly. Other functions of TRBP include promoting cellular growth and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential regulation of ATP hydrolysis of RIG-I-like receptors by transactivation response RNA-binding protein.

Bioscience reports

Chunhaphinyokul B,Hosokai E,Miyamoto M,Komuro A

Fri May 05 00:00:00 EDT 2023

Human Cytomegalovirus UL24 and UL43 Cooperate to Modulate the Expression of Immunoregulatory UL16 Binding Protein 1.

Viral immunology

Sleman S,Hao H,Najmuldeen H,Jalal P,Saeed N,Othman D,Qian Z

Sat Oct 01 00:00:00 EDT 2022

Human Cytomegalovirus UL24 and UL43 Cooperate to Modulate the Expression of Immunoregulatory UL16 Binding Protein 1.

Viral immunology

Sleman S,Hao H,Najmuldeen H,Jalal P,Saeed N,Othman D,Qian Z

Sat Oct 01 00:00:00 EDT 2022

TARBP2-stablized SNHG7 regulates blood-brain barrier permeability by acting as a competing endogenous RNA to miR-17-5p/NFATC3 in Aβ-microenvironment.

Cell death & disease

Ning H,Zhang L,Zhu B,Zhou X,Zhang T,Ma T

Fri May 13 00:00:00 EDT 2022

LINC01526 Promotes Proliferation and Metastasis of Gastric Cancer by Interacting with TARBP2 to Induce GNG7 mRNA Decay.

Cancers

Zhou JY,Liu JY,Tao Y,Chen C,Liu SL

Sun Oct 09 00:00:00 EDT 2022

LINC01526 Promotes Proliferation and Metastasis of Gastric Cancer by Interacting with TARBP2 to Induce GNG7 mRNA Decay.

Cancers

Zhou JY,Liu JY,Tao Y,Chen C,Liu SL

Sun Oct 09 00:00:00 EDT 2022

Dicer-mediated miR-200b expression contributes to cell migratory/invasive abilities and cancer stem cells properties of breast cancer cells.

Aging

Hsu TW,Chen HA,Liao PH,Su YH,Chiu CF,Huang CY,Lin YJ,Hung CC,Yeh MH,Sung SY,Su CM

Mon Aug 08 00:00:00 EDT 2022

LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.

Journal of experimental & clinical cancer research : CR

Li D,Shen L,Zhang X,Chen Z,Huang P,Huang C,Qin S

Fri Dec 02 00:00:00 EST 2022

Hypoxia regulates overall mRNA homeostasis by inducing Met¹-linked linear ubiquitination of AGO2 in cancer cells.

Nature communications

Zhang H,Zhao X,Guo Y,Chen R,He J,Li L,Qiang Z,Yang Q,Liu X,Huang C,Lu R,Fang J,Cao Y,Huang J,Wang Y,Huang J,Chen GQ,Cheng J,Yu J

Mon Sep 13 00:00:00 EDT 2021

Amyloid peptide exerts a rapid induction of Dicer1 protein in neuron via reducing phosphorylation.

Neurochemistry international

Wang Y,Xiu X,Wu S

Wed Dec 01 00:00:00 EST 2021

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Journal of medicinal chemistry

Zhou Z,Li Y,Ma X,Cao B,Peng T,Sheng Y,Peng H,Li R,Cao Y,Xi R,Li F,Wang M,Sun H,Zhang G,Zhang H,Hu K,Xiao W,Wang F

Thu Jun 10 00:00:00 EDT 2021

Loss of TARBP2 Drives the Progression of Hepatocellular Carcinoma via miR-145-SERPINE1 Axis.

Frontiers in oncology

Li LM,Chen C,Ran RX,Huang JT,Sun HL,Zeng C,Zhang Z,Zhang W,Liu SM

Fri Sep 24 00:00:00 EDT 2021

Dietary daidzein inhibits hepatitis C virus replication by decreasing microRNA-122 levels.

Virus research

He Y,Huang M,Tang C,Yue Y,Liu X,Zheng Z,Dong H,Liu D

Tue Jun 01 00:00:00 EDT 2021

Evidence of A Negative Feedback Network Between TDP-43 and miRNAs Dependent on TDP-43 Nuclear Localization.

Journal of molecular biology

Hawley ZCE,Campos-Melo D,Strong MJ

Fri Dec 04 00:00:00 EST 2020

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Nature

Ringeard M,Marchand V,Decroly E,Motorin Y,Bennasser Y

Tue Jan 01 00:00:00 EST 2019

Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.

Molecular cell

Fish L,Navickas A,Culbertson B,Xu Y,Nguyen HCB,Zhang S,Hochman M,Okimoto R,Dill BD,Molina H,Najafabadi HS,Alarcón C,Ruggero D,Goodarzi H

Thu Sep 05 00:00:00 EDT 2019

Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.

Molecular cell

Fish L,Navickas A,Culbertson B,Xu Y,Nguyen HCB,Zhang S,Hochman M,Okimoto R,Dill BD,Molina H,Najafabadi HS,Alarcón C,Ruggero D,Goodarzi H

Thu Sep 05 00:00:00 EDT 2019

A biosensor for MAPK-dependent Lin28 signaling.

Molecular biology of the cell

Oldach LM,Gorshkov K,Mills WT,Zhang J,Meffert MK

Tue May 15 00:00:00 EDT 2018

A biosensor for MAPK-dependent Lin28 signaling.

Molecular biology of the cell

Oldach LM,Gorshkov K,Mills WT,Zhang J,Meffert MK

Tue May 15 00:00:00 EDT 2018

A biosensor for MAPK-dependent Lin28 signaling.

Molecular biology of the cell

Oldach LM,Gorshkov K,Mills WT,Zhang J,Meffert MK

Tue May 15 00:00:00 EDT 2018

A biosensor for MAPK-dependent Lin28 signaling.

Molecular biology of the cell

Oldach LM,Gorshkov K,Mills WT,Zhang J,Meffert MK

Tue May 15 00:00:00 EDT 2018

A Rapid Induction Mechanism for Lin28a in Trophic Responses.

Molecular cell

Amen AM,Ruiz-Garzon CR,Shi J,Subramanian M,Pham DL,Meffert MK

Thu Feb 02 00:00:00 EST 2017

A Rapid Induction Mechanism for Lin28a in Trophic Responses.

Molecular cell

Amen AM,Ruiz-Garzon CR,Shi J,Subramanian M,Pham DL,Meffert MK

Thu Feb 02 00:00:00 EST 2017

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Nucleic acids research

Warner MJ,Bridge KS,Hewitson JP,Hodgkinson MR,Heyam A,Massa BC,Haslam JC,Chatzifrangkeskou M,Evans GJ,Plevin MJ,Sharp TV,Lagos D

Wed Nov 16 00:00:00 EST 2016

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Nucleic acids research

Warner MJ,Bridge KS,Hewitson JP,Hodgkinson MR,Heyam A,Massa BC,Haslam JC,Chatzifrangkeskou M,Evans GJ,Plevin MJ,Sharp TV,Lagos D

Wed Nov 16 00:00:00 EST 2016

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Nucleic acids research

Warner MJ,Bridge KS,Hewitson JP,Hodgkinson MR,Heyam A,Massa BC,Haslam JC,Chatzifrangkeskou M,Evans GJ,Plevin MJ,Sharp TV,Lagos D

Wed Nov 16 00:00:00 EST 2016

S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.

Nucleic acids research

Warner MJ,Bridge KS,Hewitson JP,Hodgkinson MR,Heyam A,Massa BC,Haslam JC,Chatzifrangkeskou M,Evans GJ,Plevin MJ,Sharp TV,Lagos D

Wed Nov 16 00:00:00 EST 2016

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Biochemical and biophysical research communications

Komuro A,Homma Y,Negoro T,Barber GN,Horvath CM

Fri Nov 11 00:00:00 EST 2016

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Biochemical and biophysical research communications

Komuro A,Homma Y,Negoro T,Barber GN,Horvath CM

Fri Nov 11 00:00:00 EST 2016

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.

Nature communications

Chen C,Zhu C,Huang J,Zhao X,Deng R,Zhang H,Dou J,Chen Q,Xu M,Yuan H,Wang Y,Yu J

Thu Nov 19 00:00:00 EST 2015

SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.

Nature communications

Chen C,Zhu C,Huang J,Zhao X,Deng R,Zhang H,Dou J,Chen Q,Xu M,Yuan H,Wang Y,Yu J

Thu Nov 19 00:00:00 EST 2015

Bioinformatics

Protein Aliases: PRM-1 RNA-binding protein; Protamine-1 RNA-binding protein; RISC-loading complex subunit TARBP2; RISC-loading complex subunit TARBP2 {ECO:0000255|HAMAP-Rule:MF_03034}; TAR (HIV) RNA binding protein 2; TAR (HIV) RNA-binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR (HIV-1) RNA binding protein 2; TAR RNA binding protein 2; TAR RNA-binding protein 2; TARBP2; trans-activation responsive RNA-binding protein; Trans-activation-responsive RNA-binding protein

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Gene Aliases: LOQS; Prbp; TARBP2; TRBP; TRBP1; TRBP2

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UniProt ID: (Human) Q15633, (Mouse) P97473, (Rat) Q3SWU0

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Entrez Gene ID: (Human) 6895, (Mouse) 21357, (Rat) 363006

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Function(s)

double-stranded RNA binding protein binding enzyme binding siRNA binding miRNA binding pre-mRNA binding protein homodimerization activity protein N-terminus binding pre-miRNA binding RNA binding molecular_function

Process(es)

negative regulation of protein kinase activity spermatid development single fertilization production of siRNA involved in RNA interference targeting of mRNA for destruction involved in RNA interference gene silencing by RNA pre-miRNA processing siRNA loading onto RISC involved in RNA interference production of miRNAs involved in gene silencing by miRNA multicellular organism growth miRNA loading onto RISC involved in gene silencing by miRNA positive regulation of viral genome replication positive regulation of translation regulation of viral transcription negative regulation of defense response to virus by host regulation of translation biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRBP Polyclonal Antibody

TRBP Antibody (15753-1-AP) in ICC/IF

Product Details

15753-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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