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TRIM27 Antibody (CF804048) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM27 (RC202184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY401952 (100 µg) and LC401952 (20 µg) can be purchased separately from OriGene.
Product Details
CF804048
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2F9
Immunogen
Full length human recombinant protein of human TRIM27 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ret finger protein; RFP transforming protein; RING finger protein 76; tripartite motif protein TRIM27; tripartite motif-containing 27; Tripartite motif-containing protein 27; Zinc finger protein RFP
Gene Aliases: RFP; RNF76; TRIM27
UniProt ID: (Human) P14373
Entrez Gene ID: (Human) 5987
negative regulation of transcription from RNA polymerase II promoter
negative regulation of adaptive immune response
transcription, DNA-templated
negative regulation of protein kinase activity
spermatogenesis
cell proliferation
peptidyl-tyrosine phosphorylation
negative regulation of tumor necrosis factor production
negative regulation of viral transcription
Arp2/3 complex-mediated actin nucleation
retrograde transport, endosome to Golgi
innate immune response
negative regulation of gene expression, epigenetic
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of actin nucleation
protein trimerization
protein K63-linked ubiquitination
interferon-gamma secretion
negative regulation of calcium ion import
negative regulation of interleukin-2 secretion
negative regulation of viral release from host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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