Invitrogen

TRIM28 Polyclonal Antibody

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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Datasheet
Protocols
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Product Details

PA1-852

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:500
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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human KAP-1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2219366

Product Specific Information

PA1-852 detects KAP-1 (Kruppel-associated box-associated protein 1) in human, mouse and rat samples.

PA1-852 has been successfully used in Western blot, ICC/IF and IHC (P) procedures. By Western blot, this antibody detects an ~87 kDa protein representing recombinant human KAP-1. This antibody also detects a smaller protein that is believed to be KAP-1 degradation product. In immunofluorescent applications, this antibody primarily reacts with mouse or rat and shows non-specific staining in HeLa cells.

The PA1-852 antigen is a recombinant human KAP-1.

Target Information

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28

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Gene Aliases: AA408787; KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; Tif1beta; TRIM28

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UniProt ID: (Human) Q13263, (Mouse) Q62318, (Rat) O08629

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Entrez Gene ID: (Human) 10155, (Mouse) 21849, (Rat) 116698

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Function(s)
RNA polymerase II transcription coactivator activity DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein kinase activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase binding Krueppel-associated box domain binding sequence-specific DNA binding poly(A) RNA binding chromo shadow domain binding promoter-specific chromatin binding transcription coactivator activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development epithelial to mesenchymal transition DNA repair transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation embryo implantation protein ubiquitination protein sumoylation positive regulation of transcription factor import into nucleus DNA methylation involved in embryo development innate immune response positive regulation of DNA repair negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated protein autophosphorylation protein oligomerization convergent extension involved in axis elongation embryonic placenta morphogenesis negative regulation of DNA demethylation negative regulation of viral release from host cell regulation of genetic imprinting transcription initiation from RNA polymerase II promoter Ras protein signal transduction covalent chromatin modification positive regulation of transcription from RNA polymerase II promoter positive regulation of methylation-dependent chromatin silencing
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