Invitrogen

TRIM28 Monoclonal Antibody (20C1)

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

7 Published Figures

9 References

View all (67) TRIM28 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TRIM28 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

TRIM28 Antibody (MA1-2023) in ICC/IF

Immunofluorescence analysis of TIF1 beta was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRIM28 (20C1) Mouse Monoclonal Antibody (Product # MA1-2023) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

TRIM28 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

TRIM28 Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-2023

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

20C1

Immunogen

Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2209892

Product Specific Information

MA1-2023 detects human and mouse TIF1 beta.

MA1-2023 has been used successfully in Western blot, and immunofluorescence procedures. By Western blot this antibody detects ~100 kDa protein representing TIF1 beta in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, MA1-2023 recognizes TIF1 beta in gamma irradiated HeLa cells.

The MA1-2023 immunogen is recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.

Target Information

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues.

Cancers

Shah PA,Boutros-Suleiman S,Emanuelli A,Paolini B,Levy-Cohen G,Blank M

MA1-2023 was used in ChIP Assay, Gel Shift, Immunocytochemistry, Immunoprecipitation, Western Blot, Proximity Ligation Assay to uncover SMURF2 as a novel regulator of KAP1, governing its protein expression, interactions, and functions.

Tue Mar 22 00:00:00 EDT 2022

The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues.

Cancers

Shah PA,Boutros-Suleiman S,Emanuelli A,Paolini B,Levy-Cohen G,Blank M

Tue Mar 22 00:00:00 EDT 2022

The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues.

Cancers

Shah PA,Boutros-Suleiman S,Emanuelli A,Paolini B,Levy-Cohen G,Blank M

Tue Mar 22 00:00:00 EDT 2022

The Emerging Role of E3 Ubiquitin Ligase SMURF2 in the Regulation of Transcriptional Co-Repressor KAP1 in Untransformed and Cancer Cells and Tissues.

Cancers

Shah PA,Boutros-Suleiman S,Emanuelli A,Paolini B,Levy-Cohen G,Blank M

Tue Mar 22 00:00:00 EDT 2022

lncRNA BORG:TRIM28 Complexes Drive Metastatic Progression by Inducing α6 Integrin/CD49f Expression in Breast Cancer Stem Cells.

Molecular cancer research : MCR

Parker KA,Gooding AJ,Valadkhan S,Schiemann WP

MA1-2023 was used in ChIP Assay, Gel Shift, Immunocytochemistry, Immunoprecipitation, Western Blot to implicate BORG:TRIM28 complexes as novel drivers of BCSC phenotypes in developing and progressing TNBCs.

Wed Dec 01 00:00:00 EST 2021

The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation.

Genes & development

Palangat M,Anastasakis DG,Fei DL,Lindblad KE,Bradley R,Hourigan CS,Hafner M,Larson DR

MA1-2023 was used in Immunocytochemistry-immunoflourescence to show a noncanonical function of U2AF1 whereby it can directly bind mature mNA in the cytoplasm and negatively regulate mRNA translation, contributing to cancer progression.

Wed May 01 00:00:00 EDT 2019

The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency.

Stem cell reports

Emani MR,Närvä E,Stubb A,Chakroborty D,Viitala M,Rokka A,Rahkonen N,Moulder R,Denessiouk K,Trokovic R,Lund R,Elo LL,Lahesmaa R

Published figure using TRIM28 monoclonal antibody (Product # MA1-2023) in Western Blot

Tue Mar 10 00:00:00 EDT 2015

TRIM28 is required by the mouse KRAB domain protein ZFP568 to control convergent extension and morphogenesis of extra-embryonic tissues.

Development (Cambridge, England)

Shibata M,Blauvelt KE,Liem KF,García-García MJ

Published figure using TRIM28 monoclonal antibody (Product # MA1-2023) in Western Blot

Thu Dec 01 00:00:00 EST 2011

TRIM28 is required by the mouse KRAB domain protein ZFP568 to control convergent extension and morphogenesis of extra-embryonic tissues.

Development (Cambridge, England)

Shibata M,Blauvelt KE,Liem KF,García-García MJ

Published figure using TRIM28 monoclonal antibody (Product # MA1-2023) in Western Blot

Thu Dec 01 00:00:00 EST 2011

TRIM28 is required by the mouse KRAB domain protein ZFP568 to control convergent extension and morphogenesis of extra-embryonic tissues.

Development (Cambridge, England)

Shibata M,Blauvelt KE,Liem KF,García-García MJ

Published figure using TRIM28 monoclonal antibody (Product # MA1-2023) in Western Blot

Thu Dec 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28

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Gene Aliases: AA408787; KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; Tif1beta; TRIM28

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UniProt ID: (Human) Q13263, (Mouse) Q62318

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Entrez Gene ID: (Human) 10155, (Mouse) 21849

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Function(s)

RNA polymerase II transcription coactivator activity DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein kinase activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity ubiquitin protein ligase binding Krueppel-associated box domain binding sequence-specific DNA binding poly(A) RNA binding chromo shadow domain binding promoter-specific chromatin binding transcription coactivator activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development epithelial to mesenchymal transition DNA repair transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation embryo implantation protein ubiquitination protein sumoylation positive regulation of transcription factor import into nucleus DNA methylation involved in embryo development innate immune response positive regulation of DNA repair negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated protein autophosphorylation protein oligomerization convergent extension involved in axis elongation embryonic placenta morphogenesis negative regulation of DNA demethylation negative regulation of viral release from host cell regulation of genetic imprinting transcription initiation from RNA polymerase II promoter Ras protein signal transduction covalent chromatin modification positive regulation of transcription from RNA polymerase II promoter positive regulation of methylation-dependent chromatin silencing

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TRIM28 Monoclonal Antibody (20C1)

TRIM28 Antibody (MA1-2023) in ICC/IF

Product Details

MA1-2023

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

in situ PLA (PLA)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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