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Invitrogen
TROP2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
TROP2 Antibody (PA5-47030) in ICC/IF
Immunofluorescence analysis of TROP2 was performed using 70% confluent log phase MCF-7 cells treated with 5-aza-2-deoxycytidine (2.5 µM for 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TROP2 Polyclonal Antibody (Product # PA5-47030) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant S... View More
Product Details
PA5-47030
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2 Thr88-Thr274
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609571
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM-L1, and rhBCAM is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40kD glycoprotein, identified by monoclonal antibody GA733; Cell surface glycoprotein Trop-2; cell surface glycoprotein TROP2; epithelial glycoprotein-1; GA733; gastrointestinal tumor-associated antigen GA7331; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331; TACD2; truncated TACSTD2; Tumor-associated calcium signal transducer 2
Gene Aliases: EGP-1; EGP1; GA733-1; GA7331; GP50; M1S1; TACSTD2; TROP2
UniProt ID: (Human) P09758
Entrez Gene ID: (Human) 4070
cell surface receptor signaling pathway
visual perception
cell proliferation
negative regulation of epithelial cell migration
regulation of epithelial cell proliferation
negative regulation of stress fiber assembly
ureteric bud morphogenesis
negative regulation of branching involved in ureteric bud morphogenesis
cell-cell adhesion
negative regulation of substrate adhesion-dependent cell spreading
negative regulation of ruffle assembly
negative regulation of cell motility
positive regulation of stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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