Invitrogen

TRPA1 Polyclonal Antibody

3 Published Figures

5 References

View all (29) TRPA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (3)

TRPA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

TRPA1 Antibody (PA1-46159) in ICC/IF

Immunocytochemistry analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

TRPA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-46159

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:250

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Guinea pig, Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminus (residues 1-100) of the human TRPA1 protein.[Swis-Prot #075762]

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2209939

Product Specific Information

The target sequence has 69% sequence homology with rat.

Suggested positive control: human brain membrane fraction.

Target Information

TRPA1 is a TRP-related channel that responds to cold temperatures and pungent compounds and plays a role in both nociceptor and hair cell transduction. TRPA1 is a transformation -associated gene product in lung epithelia, whereas its protein distribution is primarily restricted to sensory neurons. Blocking TRPA1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage. It is now known that TRPA1 protein is also widely expressed outside of the CNS and is dys-regulated during oncogenic transformation. The structure of TRPA1 is highly related to both the protein ankyrin and transmembrane proteins. The specific function of TRPA1 has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Allyl Isothiocianate Induces Ca²⁺ Signals and Nitric Oxide Release by Inducing Reactive Oxygen Species Production in the Human Cerebrovascular Endothelial Cell Line hCMEC/D3.

Cells

Berra-Romani R,Brunetti V,Pellavio G,Soda T,Laforenza U,Scarpellino G,Moccia F

PA1-46159 was used in Western Blotting to suggest that AITC could be exploited to restore NO signalling and restore CBF in brain disorders that feature neurovascular dysfunction.

Tue Jun 27 00:00:00 EDT 2023

Allyl Isothiocianate Induces Ca²⁺ Signals and Nitric Oxide Release by Inducing Reactive Oxygen Species Production in the Human Cerebrovascular Endothelial Cell Line hCMEC/D3.

Cells

Berra-Romani R,Brunetti V,Pellavio G,Soda T,Laforenza U,Scarpellino G,Moccia F

PA1-46159 was used in Western Blotting to suggest that AITC could be exploited to restore NO signalling and restore CBF in brain disorders that feature neurovascular dysfunction.

Tue Jun 27 00:00:00 EDT 2023

Transient receptor potential ankyrin 1 (TRPA1) mediates reactive oxygen species-induced Ca²⁺ entry, mitochondrial dysfunction, and caspase-3/7 activation in primary cultures of metastatic colorectal carcinoma cells.

Cell death discovery

Faris P,Rumolo A,Pellavio G,Tanzi M,Vismara M,Berra-Romani R,Gerbino A,Corallo S,Pedrazzoli P,Laforenza U,Montagna D,Moccia F

PA1-46159 was used in Western Blotting to find that TRPA1 channel protein is up-regulated and mediates enhanced hydrogen peroxide (H2O2)-induced Ca2+ entry in mCRC cells as compared to non-neoplastic control cells.

Sat Jul 01 00:00:00 EDT 2023

Cell death discovery

Faris P,Rumolo A,Pellavio G,Tanzi M,Vismara M,Berra-Romani R,Gerbino A,Corallo S,Pedrazzoli P,Laforenza U,Montagna D,Moccia F

Sat Jul 01 00:00:00 EDT 2023

Interleukin 1 beta-induced calcium signaling via TRPA1 channels promotes mitogen-activated protein kinase-dependent mesangial cell proliferation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Soni H,Kumar R,Kanthakumar P,Adebiyi A

PA1-46159 was used in Western Blotting to show that the beta isoform of IL-1 (IL-1β) is a potent inducer of IL-1 type I receptor-dependent Ca2+ entry in mouse Glomerular mesangial cell.

Thu Jul 01 00:00:00 EDT 2021

Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Scientific reports

Cojocaru F,Şelescu T,Domocoş D,Măruţescu L,Chiritoiu G,Chelaru NR,Dima S,Mihăilescu D,Babes A,Cucu D

PA1-46159 was used in Western Blotting to support the idea of channel-dependent and independent role for TRPA1 in tumoral processes.

Thu Jan 21 00:00:00 EST 2021

Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Scientific reports

Cojocaru F,Şelescu T,Domocoş D,Măruţescu L,Chiritoiu G,Chelaru NR,Dima S,Mihăilescu D,Babes A,Cucu D

PA1-46159 was used in Western Blotting to support the idea of channel-dependent and independent role for TRPA1 in tumoral processes.

Thu Jan 21 00:00:00 EST 2021

Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice.

The Journal of experimental biology

Wen J,Bo T,Zhang X,Wang Z,Wang D

PA1-46159 was used in Western Blotting to test the hypothesis that thermo-TRPs and gut microbiota are involved in reducing diet-induced obesity (DIO) during low temperature exposure.

Thu Jun 04 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ANKTM1; ankyrin-like with transmembrane domains 1; Ankyrin-like with transmembrane domains protein 1; ion channel; drTRPA1; nompC; p120; Transformation-sensitive protein p120; transient receptor potential A1; Transient receptor potential cation channel subfamily A member 1; transient receptor potential cation channel, subfamily A, member 1; TRPA-1; Wasabi receptor

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Gene Aliases: ANKTM1; FEPS; TRPA1; TRPA1b

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UniProt ID: (Human) O75762, (Mouse) Q8BLA8, (Rat) Q6RI86

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Entrez Gene ID: (Human) 8989, (Mouse) 277328, (Guinea pig) 100526649, (Rat) 312896, (Zebrafish) 474351

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Function(s)

calcium channel activity channel activity calcium-release channel activity temperature-gated cation channel activity ion channel activity protein binding mechanically-gated ion channel activity ligand-gated ion channel activity scaffold/adaptor protein

Process(es)

ion transport cell surface receptor signaling pathway response to cold response to organic substance response to organic cyclic compound sensory perception of pain response to drug response to hydrogen peroxide response to pain thermoception detection of mechanical stimulus involved in sensory perception of pain detection of chemical stimulus involved in sensory perception of pain release of sequestered calcium ion into cytosol protein homotetramerization calcium ion transmembrane transport transport calcium ion transport ion transmembrane transport response to stimulus detection of chemical stimulus detection of temperature stimulus involved in thermoception detection of mechanical stimulus involved in sensory perception transmembrane transport response to fatty acid

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRPA1 Polyclonal Antibody

TRPA1 Antibody (PA1-46159) in ICC/IF

Product Details

PA1-46159

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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