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TRPM8 Antibody (OST00181W-100UL) in IHC (P)
IHC-P on paraffin sections of mouse testis. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 mL 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chr... View More
Product Details
OST00181W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from rat TRPM8 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous with the corresponding sequence in mouse.
Specificity of this antibody: TRPM8.
Target Information
TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. TRPM8 is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. TRPM8 is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. TRPM8 has an expression pattern within normal human tissues that is mostly restricted to prostate epithelial cells. TRPM8 mRNA expression is at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, TRPM8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding TRPM8 were hardly detected or not detected in the corresponding normal human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cold menthol receptor 1; cold/menthol receptor 1; Long transient receptor potential channel 6; LTrpC-6; MGC2849; Transient receptor potential cation channel subfamily M member 8; Transient receptor potential p8; transient receptor potential-p8; TRP ion channel TRPM8; Trp-p8
Gene Aliases: CMR1; LTrpC-6; LTRPC6; Trp-p8; Trpm8; TRPP8
UniProt ID: (Rat) Q8R455, (Mouse) Q8R4D5
Entrez Gene ID: (Rat) 171384, (Mouse) 171382
calcium ion transport
cellular calcium ion homeostasis
response to temperature stimulus
response to cold
detection of temperature stimulus
thermoception
protein homotetramerization
protein homotrimerization
calcium ion transmembrane transport
transport
ion transport
cation transport
ion transmembrane transport
response to stimulus
sensory perception of temperature stimulus
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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