Invitrogen

TRPV1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

16 References

View all (41) TRPV1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

TRPV1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 14

TRPV1 Antibody (PA1-748) in IHC (F)

Frozen human skin tissue sections were fixed with paraformaldehyde and stained with PA1-748 (1:250) followed by a biotin-conjugated donkey anti-rabbit IgG secondary antibody and streptavidin-HRP/tyrimide-fluorescent detection system. Vanilloid Receptor 1 staining is shown in red. Data courtesy of the Innovators Program.

TRPV1 Antibody in Immunohistochemistry (IHC)

TRPV1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

TRPV1 Antibody — Figure 5 Evaluation of the effect of capsaicin on SIRT6-mediated OD. The hMSCs were transfected with SIRT6 overexpression vector, and treated with 10 muM capsaicin for 12 h. ( A ) ELISA was utilized to detect released CGRP. ( B ) Western blot analysis was performed to detect SIRT6 and TRPV1 protein levels. ( C ) Levels of the osteoblastic markers OPN , OCN , BSP , RUNX2 , and ALP were determined via qPCR for each group on day 14 after induction. ( D ) The activity of ALP was detected in each group in OD. ( E ) AR staining was performed to detect matrix mineralization of hMSCs between different treatments on day 14 following induction. N=3. One-way ANOVA was used for comparative analyses of the data in ( A, C, D ). * P<0.05 vs. indicated groups. {TM}

Product Details

PA1-748

Applications

Tested Dilution

Publications

Western Blot (WB)

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues T(7) D L G A A A D P L Q K D T C(21) of human VR1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2209010

Product Specific Information

PA1-748 detects vanilloid receptor 1 (VR1) from human bladder and skin.

PA1-748 has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunohistochemical staining of VR1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of VR1 in human prostatic urethra using PA1-748 results in fibrous staining within the epithelial cells.

The PA1-748 immunogen is a synthetic peptide corresponding to residues T(7) D L G A A A D P L Q K D T C(21) of human VR1. PA1-748 immunizing peptide (Cat. # PEP-202) is available for use in neutralization and control experiments.

Target Information

TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1) is a receptor for capsaicin, the main pungent ingredient in hot chili peppers, and elicits a sensation of burning pain by selectively activating sensory neurons. TRPV1 is a non-selective cation channel that is structurally related to members of the TRP family of ion channels such as TRPC3 and TRPC6. TRPV1 is permeable to calcium, magnesium, and monovalent cations of sodium and potassium. Structurally, TRPV1 contains 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. TRPV1 is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. TRPV1 seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. The expression of TRPV1 appears to be limited to neurons within the sensory ganglia. Four transcript variants encoding TRPV1 with variations in th 5' UTR sequence have been described. Diseases associated with TRPV1 include Irritable Bowel Syndrome (IBS) and Bladder Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development of a functional human induced pluripotent stem cell-derived nociceptor MEA system as a pain model for analgesic drug testing.

Frontiers in cell and developmental biology

Nimbalkar S,Guo X,Colón A,Jackson M,Akanda N,Patel A,Grillo M,Hickman JJ

PA1-748 was used in Immunocytochemistry to propose a human-based functional nociceptive system, providing a valuable platform for investigating pathological pain and for evaluating effective analgesics in the search of opioid substitutes.

Tue Mar 21 00:00:00 EDT 2023

Convergence of peptidergic and non-peptidergic protein markers in the human dorsal root ganglion and spinal dorsal horn.

The Journal of comparative neurology

Shiers SI,Sankaranarayanan I,Jeevakumar V,Cervantes A,Reese JC,Price TJ

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Immunohistochemistry

Thu Jul 01 00:00:00 EDT 2021

Inhibition of carrageenan-induced dental inflammatory responses owing to decreased TRPV1 activity by Dexmedetomidine.

Journal of inflammation (London, England)

Lv G,Zhu G,Xu M,Gao X,Xiao Q

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Western Blot

Thu Apr 14 00:00:00 EDT 2022

Inhibition of carrageenan-induced dental inflammatory responses owing to decreased TRPV1 activity by Dexmedetomidine.

Journal of inflammation (London, England)

Lv G,Zhu G,Xu M,Gao X,Xiao Q

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Western Blot

Thu Apr 14 00:00:00 EDT 2022

Prognostic Significance of Transient Receptor Potential Vanilloid Type 1 (TRPV1) and Phosphatase and Tension Homolog (PTEN) in Epithelial Ovarian Cancer.

Cancer genomics & proteomics

Han GH,Chay DB,Nam S,Cho H,Chung JY,Kim JH

PA1-748 was used in Western Blotting to investigate the significance of TRPV1 and correlation with phosphatase and tension homolog (PTEN) in epithelial ovarian cancer (EOC).

Mon Nov 23 00:00:00 EST 2020

Inhibitory Effect of Sirtuin6 (SIRT6) on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells.

Medical science monitor : international medical journal of experimental and clinical research

Xiao F,Zhou Y,Liu Y,Xie M,Guo G

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Western Blot

Fri Nov 08 00:00:00 EST 2019

Sirtuin6 inhibits c-triggered inflammation through TLR4 abrogation regulated by ROS and TRPV1/CGRP.

Journal of cellular biochemistry

Zhang R,Li H,Guo Q,Zhang L,Zhu J,Ji J

PA1-748 was used in Western Blotting to indicate that SIRT6 serves as a potential therapeutic target to alleviate acne inflammation.

Thu Nov 01 00:00:00 EDT 2018

Activation of endogenous TRPV1 fails to induce overstimulation-based cytotoxicity in breast and prostate cancer cells but not in pain-sensing neurons.

Biochimica et biophysica acta

Pecze L,Jósvay K,Blum W,Petrovics G,Vizler C,Oláh Z,Schwaller B

PA1-748 was used in western blot to assess the failure to induce overstimulation-based cytotoxicity in breast and prostate cancer cells and not pain-sensing neurons after activation of endogenous TRPV1

Mon Aug 01 00:00:00 EDT 2016

Vanilloids induce oral cancer apoptosis independent of TRPV1.

Oral oncology

Gonzales CB,Kirma NB,De La Chapa JJ,Chen R,Henry MA,Luo S,Hargreaves KM

Thu May 01 00:00:00 EDT 2014

Vanilloids induce oral cancer apoptosis independent of TRPV1.

Oral oncology

Gonzales CB,Kirma NB,De La Chapa JJ,Chen R,Henry MA,Luo S,Hargreaves KM

Thu May 01 00:00:00 EDT 2014

The neurochemical changes in the innervation of human colonic mesenteric and submucosal blood vessels in ulcerative colitis and Crohn's disease.

Neurogastroenterology and motility

de Fontgalland D,Brookes SJ,Gibbins I,Sia TC,Wattchow DA

PA1-748 was used in immunohistochemistry to study colonic mesenteric and submucosal blood vessel ennervation in patients with ulcerative colitis and Crohn's disease

Thu May 01 00:00:00 EDT 2014

TRPV1 and TRPA1 stimulation induces MUC5B secretion in the human nasal airway in vivo.

Clinical physiology and functional imaging

Alenmyr L,Herrmann A,Högestätt ED,Greiff L,Zygmunt PM

PA1-748 was used in immunohistochemistry to study the effect of TRPV1 and TRPA1 on MUC5B secretion in the human nasal airway in vivo

Tue Nov 01 00:00:00 EDT 2011

Transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 immunoreactive nerve fibers in human skin from individuals with and without Norrbottnian congenital insensitivity to pain.

Neuroscience

Axelsson HE,Minde JK,Sonesson A,Toolanen G,Högestätt ED,Zygmunt PM

PA1-748 was used in immunohistochemistry to study the role of transient receptor potential vanilloid 1, vanilloid 2 and melastatin 8 in nociceptive primary sensory neurons in human skin

Tue Sep 15 00:00:00 EDT 2009

Human keratinocytes are vanilloid resistant.

PloS one

Pecze L,Szabó K,Széll M,Jósvay K,Kaszás K,Kúsz E,Letoha T,Prorok J,Koncz I,Tóth A,Kemény L,Vizler C,Oláh Z

PA1-748 was used in western blot to study the mechanism of the vanilloid resistance in human keratinocytes

Thu Dec 18 00:00:00 EST 2008

Human keratinocytes are vanilloid resistant.

PloS one

Pecze L,Szabó K,Széll M,Jósvay K,Kaszás K,Kúsz E,Letoha T,Prorok J,Koncz I,Tóth A,Kemény L,Vizler C,Oláh Z

PA1-748 was used in western blot to study the mechanism of the vanilloid resistance in human keratinocytes

Thu Dec 18 00:00:00 EST 2008

The distribution of sensory fibers immunoreactive for the TRPV1 (capsaicin) receptor in the human prostate.

European urology

Dinis P,Charrua A,Avelino A,Nagy I,Quintas J,Ribau U,Cruz F

PA1-748 was used in Immunofluorescence in immunohistochemistry on frozen tissues to determine the distribution of sensory fibres immunoreactive to the pain receptor TRPV1 in the human prostate.

Fri Jul 01 00:00:00 EDT 2005

Deletion of vanilloid receptor 1-expressing primary afferent neurons for pain control.

The Journal of clinical investigation

Karai L,Brown DC,Mannes AJ,Connelly ST,Brown J,Gandal M,Wellisch OM,Neubert JK,Olah Z,Iadarola MJ

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2004

Deletion of vanilloid receptor 1-expressing primary afferent neurons for pain control.

The Journal of clinical investigation

Karai L,Brown DC,Mannes AJ,Connelly ST,Brown J,Gandal M,Wellisch OM,Neubert JK,Olah Z,Iadarola MJ

Published figure using TRPV1 polyclonal antibody (Product # PA1-748) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: Capsaicin receptor; DKFZp434K0220; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; transient receptor potential cation channel, subfamily V, member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; TrpV1; Vanilloid receptor; Vanilloid receptor 1; vanilloid receptor subtype 1

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Gene Aliases: TRPV1; VR1

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UniProt ID: (Human) Q9NQ74

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Entrez Gene ID: (Human) 7442

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Function(s)

transmembrane signaling receptor activity excitatory extracellular ligand-gated ion channel activity calcium channel activity calmodulin binding ATP binding calcium-release channel activity phosphoprotein binding ion channel

Process(es)

transport cell surface receptor signaling pathway chemosensory behavior thermoception release of sequestered calcium ion into cytosol calcium ion transmembrane transport cellular response to alkaloid cellular response to ATP

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TRPV1 Polyclonal Antibody

TRPV1 Antibody (PA1-748) in IHC (F)

Product Details

PA1-748

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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