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TRPV4 Antibody (OST00185W-100UL) in IHC (P)
IHC on paraffin sections of rat testis tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris... View More
Product Details
OST00185W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from the 4th cytoplasmic loop of rat TRPV4 (VRL-2, VRL2) conjugated to an immunogenic carrier protein was used as the immunogen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: TRPV4.
Target Information
TRPV4 (Transient Receptor Potential Cation Channel Subfamily V Member 4) is a cation-selective channel, activated in response to systemic osmotic pressure. Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. TRPV4 is a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The TRPV4 protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in the TRPV4 gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Diseases associated with TRPV4 include Metatropic Dysplasia and Spondylometaphyseal Dysplasia, Kozlowski Type. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Osm-9-like TRP channel 4; osmosensitive transient receptor potential channel 4; OTRPC4; Transient receptor potential cation channel subfamily V member 4; transient receptor potential cation channel, subfamily 5, member 4; TrpV4; Vanilloi; vanilloid receptor-related osmotically activated channel (Vroac); Vanilloid receptor-related osmotically-activated channel; VR-OAC
Gene Aliases: Otrpc4; Trpv4; Vroac
UniProt ID: (Rat) Q9ERZ8
Entrez Gene ID: (Rat) 66026
calcium ion transport
cellular calcium ion homeostasis
response to osmotic stress
hypotonic response
actin filament organization
cell-cell junction assembly
positive regulation of cytosolic calcium ion concentration
osmosensory signaling pathway
negative regulation of neuron projection development
vasopressin secretion
positive regulation of microtubule depolymerization
actin cytoskeleton reorganization
cellular response to heat
hyperosmotic salinity response
cortical microtubule organization
microtubule polymerization
regulation of response to osmotic stress
calcium ion import
calcium ion transmembrane transport
cellular response to osmotic stress
cellular hypotonic response
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