Invitrogen

TSC1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 References

View all (42) TSC1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Advanced Verification (2)

TSC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

TSC1 Antibody (PA5-18506) in IHC (P)

Immunohistochemistry analysis of TSC1 in human uterus. Samples were incubated with TSC1 polyclonal antibody (Product # PA5-18506) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Product Image Gallery

TSC1 Antibody — Figure 1 Tsc1 ablation results in OL differentiation and maturation defects. ( a ) Immunostaining for Tsc1 in OPCs (A2B5 + ), differentiating and maturating OLs (CNP + ), terminally differentiated OL (MBP + ) induced by treatment with T3 for 0, 1 and 3 days (left to right), respectively. Scale bar, 10 mum. ( b ) Expression of Tsc1 and Tsc2 assessed by western blot in OPCs treated with T3 for 0, 1 and 3 days. beta-actin was used as loading control. ( c ) Schematic diagram of Cre-mediated excision of the floxed Tsc1 exons 17 and 18, a region of the gene that encodes an essential coiled-coil domain. ( d ) Expression of Tsc1 and Tsc2 in the corpus callosum of control and Tsc1 cKO mice at P14 was examined by western blot. ( e ) Primary OPCs from control and Tsc1 cKO mice were immunostained with antibodies to Tsc1 and Sox10. Scale bar, 10 mum. ( f ) The optic nerve of Tsc1 cKO mice at P12 appears translucent compared with the control. ( g - i ) Expression of MBP and Plp1 mRNAs was examined by in situ hybridization in the ( g , h ) cortex and ( i ) spinal cord of Tsc1 cKO and control mice at indicated ages. Scale bar, 100 mum. ( j , k ) MBP immunostaining in the ( j ) cerebellum and ( k ) cerebral cortex of control, and Tsc1 cKO at P28 and P67. Scale bar, 100 mum. Arrows indicate white matter tracts. ( l ) Expression of MBP in the corpus callosum of control and Tsc1 cKO mice at P14 was examined by western blot. beta-actin was used as loading control. {KO}

Product Details

PA5-18506

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.3 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (QLHIMDYNETHHEH) corresponding to the C-terminus amino acids of TSC1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10985558

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The TSC1-mTOR-PLK axis regulates the homeostatic switch from Schwann cell proliferation to myelination in a stage-specific manner.

Glia

Jiang M,Rao R,Wang J,Wang J,Xu L,Wu LM,Chan JR,Wang H,Lu QR

PA5-18506 was used in Immunohistochemistry to report that tuberous sclerosis complex 1 (TSC1), a negative regulator of mTOR activity, establishes a stage-dependent program for Schwann cell lineage progression and myelination by controlling cell proliferation and myelin homeostasis.

Sat Sep 01 00:00:00 EDT 2018

Regulation of PERK-eIF2α signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes.

Nature communications

Jiang M,Liu L,He X,Wang H,Lin W,Wang H,Yoon SO,Wood TL,Lu QR

PA5-18506 was used in immunocytochemistry and western blot to study the control of homoeostasis and survival of myelinating oligodendrocytes by regulation of PERK-eIF2alpha signaling by tuberous sclerosis complex-1

Fri Jul 15 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; tumor suppressor

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Gene Aliases: KIAA0243; LAM; TSC; TSC1

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UniProt ID: (Human) Q92574

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Entrez Gene ID: (Human) 7248

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Function(s)

protein binding GTPase regulator activity GTPase activating protein binding protein N-terminus binding chaperone binding

Process(es)

kidney development neural tube closure regulation of cell-matrix adhesion adaptive immune response rRNA export from nucleus regulation of translation potassium ion transport cell cycle arrest cell-matrix adhesion negative regulation of cell proliferation adult locomotory behavior negative regulation of macroautophagy negative regulation of translation hippocampus development cerebral cortex development cell projection organization negative regulation of TOR signaling response to insulin myelination memory T cell differentiation regulation of phosphoprotein phosphatase activity negative regulation of cell size regulation of protein kinase activity glucose import negative regulation of insulin receptor signaling pathway synapse organization protein stabilization protein heterooligomerization regulation of stress fiber assembly regulation of cell cycle positive regulation of focal adhesion assembly cardiac muscle cell differentiation activation of GTPase activity cellular response to oxygen-glucose deprivation regulation of neuron death

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TSC1 Polyclonal Antibody

TSC1 Antibody (PA5-18506) in IHC (P)

Product Details

PA5-18506

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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