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Invitrogen
TSC2 Recombinant Rabbit Monoclonal Antibody (ARC0019)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
TSC2 Antibody (MA5-35293) in ICC/IF
Immunofluorescence analysis of TSC2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TSC2 Recombinant Rabbit Monoclonal Antibody (ARC0019) (Product # MA5-35293) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More
Product Details
MA5-35293
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0019
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1700-1807 of human Tuberin (P49815)
Conjugate
Form
Liquid
Concentration
0.52 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849195
Product Specific Information
Immunogen sequence: AKIVSDRNLP FVARQMALHA NMASQVHHSR SNPTDIYPSK WIARLRHIKR LRQRICEEAA YSNPSLPLVH PPSHSKAPAQ TPAEPTPGYE VGQRKRLISS VEDFTEFV
Target Information
TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; Tuberin; Tuberous sclerosis 2 protein
Gene Aliases: LAM; PPP1R160; TSC2; TSC4
UniProt ID: (Human) P49815
Entrez Gene ID: (Human) 7249
neural tube closure
negative regulation of protein kinase activity
protein import into nucleus
endocytosis
cell cycle arrest
heart development
protein localization
negative regulation of cell proliferation
negative regulation of phosphatidylinositol 3-kinase signaling
viral process
vesicle-mediated transport
regulation of endocytosis
negative regulation of Wnt signaling pathway
negative regulation of TOR signaling
protein kinase B signaling
positive regulation of GTPase activity
regulation of insulin receptor signaling pathway
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
positive chemotaxis
regulation of small GTPase mediated signal transduction
regulation of cell cycle
negative regulation of protein kinase B signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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