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TSLP Antibody (500-P258BT-25UG) in WB
Western Blot: To detect hTSLP by Western Blot analysis TSLP Polyclonal Antibody, Biotin (Product # 500-P258BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTSLP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P258BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 15.0 kDa Recombinant Human TSLP
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930114
Product Specific Information
AA Sequence of recombinant protein: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPTAGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGLWRRFNRPLLK QQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TSLP. Anti-Human TSLP-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTSLP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TSLP (500-P258) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTSLP.
Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTSLP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P258BT-1MG will be provided as 2 x 500 µg
Target Information
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Thymic stromal lymphopoietin
Gene Aliases: TSLP
UniProt ID: (Human) Q969D9
Entrez Gene ID: (Human) 85480
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