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Invitrogen
TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 3
TSLP Receptor Antibody (46-5499-42) in Flow
Staining of human TSLP Receptor transfected cells with Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (blue histogram) or Anti-Human TSLP Receptor PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis.
Product Details
46-5499-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio1A6 (1A6)
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10719113
Product Specific Information
Description: The eBio1A6 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR). TSLPR is an approximately 50 kDa protein with significant similarity to the common gamma-chain. TSLPR complexes with IL-7R alpha (CD127) to form the high affinity receptor that binds thymic stromal-derived lymphopoietin (TSLP). Human TSLPR is expressed by monocytes and CD11c+ dendritic cells, and TSLP binding induces the expression of the Th2 cell-attracting chemokines CCL17 and CCL22. Furthermore, the TSLPR-induced activation of dendritic cells indirectly results in the increased secretion of Th cytokines IL-4, -5 and -13, which may be necessary for the regulation of CD4+ T cell homeostasis. In mice, deficiency of TSLPR has no effect on lymphocyte numbers, whereas double deficiency of TSLPR and common gamma-chain results in fewer lymphocytes than seen in mice deficient in the common gamma-chain alone. The eBio1A6 monoclonal antibody is able to cross-block binding of another anti-human TSLPR monoclonal antibody, 1D8.
Applications Reported: This eBio1A6 (1A6) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio1A6 (1A6) antibody has been pre-titrated and tested by flow cytometric analysis of TSLPR transfected cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells; co-transfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine receptor CRL2 precusor; Cytokine receptor-like 2; Cytokine receptor-like factor 2; IL-XR; P2RY8/CRLF2 fusion; Thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; TSLP receptor; WI2-87105E3.1
Gene Aliases: CRL2; CRLF2; CRLF2Y; ILXR; TSLPR
UniProt ID: (Human) Q9HC73
Entrez Gene ID: (Human) 64109
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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