Invitrogen

TUBA1A Polyclonal Antibody

4 Published Figures

7 References

View all (7) TUBA1A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (10)
Published Figures (4)

TUBA1A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

TUBA1A Antibody (PA5-22060) in ICC/IF

Immunofluorescence analysis of alpha Tubulin antibody was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-22060) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor®... View More

TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-22060

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 37 and 276 (Q71U36) of alpha Tubulin 1A

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11154084

Product Specific Information

Recommended positive controls: Jurkat, Raji, 293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH-3T3, mouse brain, rat brain, Drosophila.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (98%), Pig (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Absence of detectable SARS-CoV-2 replication in ex vivo cultured cornea and cornea-derived epithelial cells.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie

Bayyoud T,Vavouras Syrigos G,Ruetalo Buschinger N,Wude J,Businger R,Hu D,Iftner A,Thaler S,Schindler M

PA5-22060 was used in Western Blotting to study the possibility of SARS-CoV-2 to infect human corneal cells and tissues under standard corneal culture conditions using explants of COVID-19 donors and primary cornea-derived epithelial cells.

Wed Feb 01 00:00:00 EST 2023

Antibody RING-Mediated Destruction of Endogenous Proteins.

Molecular cell

Ibrahim AFM,Shen L,Tatham MH,Dickerson D,Prescott AR,Abidi N,Xirodimas DP,Hay RT

PA5-22060 was used in Western Blotting to develop a single-component system that can induce the rapid degradation of the specific endogenous protein itself.

Thu Jul 02 00:00:00 EDT 2020

Antibody RING-Mediated Destruction of Endogenous Proteins.

Molecular cell

Ibrahim AFM,Shen L,Tatham MH,Dickerson D,Prescott AR,Abidi N,Xirodimas DP,Hay RT

PA5-22060 was used in Western Blotting to develop a single-component system that can induce the rapid degradation of the specific endogenous protein itself.

Thu Jul 02 00:00:00 EDT 2020

Does butein affect adipogenesis?

Adipocyte

Hemmeryckx B,Vranckx C,Bauters D,Lijnen HR,Scroyen I

PA5-22060 was used in Western Blotting to investigate the effect of butein on preadipoycte differentiation, adipose tissue development and browning in rodents.

Sun Dec 01 00:00:00 EST 2019

Hepatoprotective Activity of InlB321/15, the HGFR Ligand of Bacterial Origin, in CCI4-Induced Acute Liver Injury Mice.

Biomedicines

Chalenko Y,Sobyanin K,Sysolyatina E,Midiber K,Kalinin E,Lavrikova A,Mikhaleva L,Ermolaeva S

Published figure using TUBA1A polyclonal antibody (Product # PA5-22060) in Western Blot

Thu Apr 11 00:00:00 EDT 2019

Differential miRNA expression profiling reveals miR-205-3p to be a potential radiosensitizer for low- dose ionizing radiation in DLD-1 cells.

Oncotarget

Andaur R,Tapia JC,Moreno J,Soto L,Armisen R,Marcelain K

Published figure using alpha Tubulin polyclonal antibody (Product # PA5-22060) in Immunofluorescence

Tue May 29 00:00:00 EDT 2018

Iron importers Zip8 and Zip14 are expressed in retina and regulated by retinal iron levels.

Experimental eye research

Sterling J,Guttha S,Song Y,Song D,Hadziahmetovic M,Dunaief JL

PA522060 was used in western blot to measure the expression of three ferrous iron importers, Dmt1, Zip8, and Zip14 in retinas

Wed Feb 01 00:00:00 EST 2017

The Gustatory Signaling Pathway and Bitter Taste Receptors Affect the Development of Obesity and Adipocyte Metabolism in Mice.

PloS one

Avau B,Bauters D,Steensels S,Vancleef L,Laermans J,Lesuisse J,Buyse J,Lijnen HR,Tack J,Depoortere I

PA5-22060 was used in western blot to investigate the role of the gustatory signaling pathway in diet-induced obesity

Mon Jul 25 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alpha 1a antibody; alpha 3 antibody; Alpha-tubulin 1; Alpha-tubulin 3; alpha-tubulin 3 antibody; Alpha-tubulin isotype M-alpha-1; B-ALPHA-1 antibody; brain-specific antibody; FLJ25113 antibody; FLJ30169; H2 ALPHA; H2-ALPHA; H2ALPHA; hum-a-tub1; hum-a-tub1 antibody; hum-a-tub2; hum-a-tub2 antibody; LIS3 antibody; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SW-cl.56; OK/SWcl.56; Talpha1 alpha-tubulin; TUBA1A antibody; TUBA3 antibody; TUBB1; TUBB5; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1A chain antibody; Tubulin alpha-3 chain; tubulin alpha-3 chain antibody; Tubulin B-alpha-1; tubulin B-alpha-1 antibody; tubulin, alpha 1; tubulin, alpha 1a; tubulin, alpha, brain-specific

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Gene Aliases: B-ALPHA-1; LIS3; Tuba-1; Tuba1; TUBA1A; TUBA3

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UniProt ID: (Human) Q71U36, (Mouse) Q5XJF8, (Rat) P68370

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Entrez Gene ID: (Human) 7846, (Mouse) 22142, (Rat) 64158

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding nucleotide binding protein heterodimerization activity tubulin

Process(es)

G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division protein polymerization metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TUBA1A Polyclonal Antibody

TUBA1A Antibody (PA5-22060) in ICC/IF

Product Details

PA5-22060

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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