Invitrogen

TUBA1A Polyclonal Antibody

9 Published Figures

13 References

View all (7) TUBA1A antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (16)
Published Figures (9)

TUBA1A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

TUBA1A Antibody (PA5-29444) in ICC/IF

Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-29544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 co... View More

TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-29444

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Fruit fly, Human, Insect, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2546920

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, HeLa cytosol fraction, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2, Drosophila.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Application of In vitro transcytosis models to brain targeted biologics.

PloS one

Deng K,Lu Y,Finnema SJ,Vangjeli K,Huang J,Huang L,Goodearl A

PA5-29444 was used in Simple Western - Size to evaluate human models including human cerebral microvascular endothelial cell line hCMEC/D3 and human colon epithelial cell line Caco-2 models.

Fri Aug 25 00:00:00 EDT 2023

Application of In vitro transcytosis models to brain targeted biologics.

PloS one

Deng K,Lu Y,Finnema SJ,Vangjeli K,Huang J,Huang L,Goodearl A

PA5-29444 was used in Simple Western - Size to evaluate human models including human cerebral microvascular endothelial cell line hCMEC/D3 and human colon epithelial cell line Caco-2 models.

Fri Aug 25 00:00:00 EDT 2023

BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms.

Oncogene

Singh A,Busacca S,Gaba A,Sheaff M,Poile C,Nakas A,Dzialo J,Bzura A,Dawson AG,Fennell DA,Fry AM

PA5-29444 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to propose that BRCA1-associated protein 1 inactivation causes mitotic defects through BRCA1-dependent and independent mechanisms.

Wed Feb 01 00:00:00 EST 2023

Aberrant survival of hippocampal Cajal-Retzius cells leads to memory deficits, gamma rhythmopathies and susceptibility to seizures in adult mice.

Nature communications

Riva M,Moriceau S,Morabito A,Dossi E,Sanchez-Bellot C,Azzam P,Navas-Olive A,Gal B,Dori F,Cid E,Ledonne F,David S,Trovero F,Bartolomucci M,Coppola E,Rebola N,Depaulis A,Rouach N,de la Prida LM,Oury F,Pierani A

PA5-29444 was used in Western Blotting to reveal that aberrant survival of a small proportion of postnatal hippocampal CRs results in cognitive deficits and epilepsy-prone phenotypes in adulthood.

Sat Mar 18 00:00:00 EDT 2023

Factor quinolinone inhibitors disrupt spindles and multiple LSF (TFCP2)-protein interactions in mitosis, including with microtubule-associated proteins.

PloS one

Yunes SA,Willoughby JLS,Kwan JH,Biagi JM,Pokharel N,Chin HG,York EA,Su KC,George K,Shah JV,Emili A,Schaus SE,Hansen U

PA5-29444 was used in Immunocytochemistry-immunofluorescence to show that FQI1 can rapidly and reversibly lead to mitotic arrest, even when added directly to mitotic cells, implying that FQI1-mediated mitotic defects are not transcriptionally based.

Fri Jun 17 00:00:00 EDT 2022

Investigation of niclosamide as a repurposing agent for skeletal muscle atrophy.

PloS one

Kim HJ,Lee JH,Kim SW,Lee SH,Jung DW,Williams DR

PA5-29444 was used in Western Blot to suggest that niclosamide may not be a suitable repurposing drug for glucocorticoid-induced skeletal muscle atrophy or cancer cachexia.

Thu Oct 28 00:00:00 EDT 2021

Investigation of niclosamide as a repurposing agent for skeletal muscle atrophy.

PloS one

Kim HJ,Lee JH,Kim SW,Lee SH,Jung DW,Williams DR

PA5-29444 was used in Western Blot to suggest that niclosamide may not be a suitable repurposing drug for glucocorticoid-induced skeletal muscle atrophy or cancer cachexia.

Thu Oct 28 00:00:00 EDT 2021

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

International journal of molecular sciences

Sakowicz A,Bralewska M,Pietrucha T,Figueras F,Habrowska-Górczyńska DE,Piastowska-Ciesielska AW,Gach A,Sakowicz B,Rybak-Krzyszkowska M,Huras H,Grzesiak M,Biesiada L

PA5-29444 was used in Western Blotting to investigates the mechanism of NFκB activation in a first trimester trophoblastic cell line (HTR8/SVneo) stimulated by a medium containing serum from preeclamptic (PE) or normotensive (C) women in hypoxic (2% O2) or normoxic (8% O2) conditions.

Wed Sep 22 00:00:00 EDT 2021

The Preeclamptic Environment Promotes the Activation of Transcription Factor Kappa B by P53/RSK1 Complex in a HTR8/SVneo Trophoblastic Cell Line.

International journal of molecular sciences

Sakowicz A,Bralewska M,Pietrucha T,Figueras F,Habrowska-Górczyńska DE,Piastowska-Ciesielska AW,Gach A,Sakowicz B,Rybak-Krzyszkowska M,Huras H,Grzesiak M,Biesiada L

Wed Sep 22 00:00:00 EDT 2021

Lithium Chloride Protects against Sepsis-Induced Skeletal Muscle Atrophy and Cancer Cachexia.

Cells

Lee JH,Kim SW,Kim JH,Kim HJ,Um J,Jung DW,Williams DR

Published figure using TUBA1A polyclonal antibody (Product # PA5-29444) in Western Blot

Mon Apr 26 00:00:00 EDT 2021

Biochemical detection of capsid in the nucleus during HIV-1 infection.

STAR protocols

Selyutina A,Diaz-Griffero F

PA5-29444 was used in Western Blotting to develop a protocol to biochemically track capsid in the nucleus during infection.

Fri Mar 19 00:00:00 EDT 2021

Factor quinolinone inhibitors alter cell morphology and motility by destabilizing interphase microtubules.

Scientific reports

Stoiber P,Scribani Rossi P,Pokharel N,Germany JL,York EA,Schaus SE,Hansen U

PA5-29444 was used in Western Blot to show that FQI1 interferes with microtubule-associated functions in interphase, specifically cell morphology and motility.

Tue Dec 07 00:00:00 EST 2021

Factor quinolinone inhibitors alter cell morphology and motility by destabilizing interphase microtubules.

Scientific reports

Stoiber P,Scribani Rossi P,Pokharel N,Germany JL,York EA,Schaus SE,Hansen U

PA5-29444 was used in Western Blot to show that FQI1 interferes with microtubule-associated functions in interphase, specifically cell morphology and motility.

Tue Dec 07 00:00:00 EST 2021

Copy number gains of the putative CRKL oncogene in laryngeal squamous cell carcinoma result in strong nuclear expression of the protein and influence cell proliferation and migration.

Scientific reports

Kostrzewska-Poczekaj M,Bednarek K,Jarmuz-Szymczak M,Bodnar M,Filas V,Marszalek A,Bartochowska A,Grenman R,Kiwerska K,Szyfter K,Giefing M

Published figure using TUBA1A polyclonal antibody (Product # PA5-29444) in Western Blot

Wed Jan 08 00:00:00 EST 2020

Copy number gains of the putative CRKL oncogene in laryngeal squamous cell carcinoma result in strong nuclear expression of the protein and influence cell proliferation and migration.

Scientific reports

Kostrzewska-Poczekaj M,Bednarek K,Jarmuz-Szymczak M,Bodnar M,Filas V,Marszalek A,Bartochowska A,Grenman R,Kiwerska K,Szyfter K,Giefing M

Published figure using TUBA1A polyclonal antibody (Product # PA5-29444) in Western Blot

Wed Jan 08 00:00:00 EST 2020

Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating.

Cell reports

Selyutina A,Persaud M,Lee K,KewalRamani V,Diaz-Griffero F

Published figure using TUBA1A polyclonal antibody (Product # PA5-29444) in Western Blot

Tue Sep 29 00:00:00 EDT 2020

Nuclear Import of the HIV-1 Core Precedes Reverse Transcription and Uncoating.

Cell reports

Selyutina A,Persaud M,Lee K,KewalRamani V,Diaz-Griffero F

Published figure using TUBA1A polyclonal antibody (Product # PA5-29444) in Western Blot

Tue Sep 29 00:00:00 EDT 2020

Severe biallelic loss-of-function mutations in nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) in two fetuses with fetal akinesia deformation sequence.

Experimental neurology

Lukacs M,Gilley J,Zhu Y,Orsomando G,Angeletti C,Liu J,Yang X,Park J,Hopkin RJ,Coleman MP,Zhai RG,Stottmann RW

PA5-29444 was used in Western Blot to investigate the effects of nicotinamide mononucleotide adenylyltransferase 2 on neuronal development.

Tue Oct 01 00:00:00 EDT 2019

Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.

Cells

Dubińska-Magiera M,Kozioł K,Machowska M,Piekarowicz K,Filipczak D,Rzepecki R

PA5-29444 was used in Immunocytochemistry-immunoflourescence to infer that the EDMD1 phenotype may be strengthened by the toxicity of truncated emerin expressed in patients with certain nonsense mutations in EMD.

Wed Mar 13 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: alpha 1a antibody; alpha 3 antibody; Alpha-tubulin 1; Alpha-tubulin 3; alpha-tubulin 3 antibody; Alpha-tubulin isotype M-alpha-1; B-ALPHA-1 antibody; brain-specific antibody; FLJ25113 antibody; FLJ30169; H2 ALPHA; H2-ALPHA; H2ALPHA; hum-a-tub1; hum-a-tub1 antibody; hum-a-tub2; hum-a-tub2 antibody; LIS3 antibody; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SW-cl.56; OK/SWcl.56; Talpha1 alpha-tubulin; TUBA1A antibody; TUBA3 antibody; TUBB1; TUBB5; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1A chain antibody; Tubulin alpha-3 chain; tubulin alpha-3 chain antibody; Tubulin B-alpha-1; tubulin B-alpha-1 antibody; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 6; tubulin, alpha, brain-specific; unnamed protein product

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Gene Aliases: B-ALPHA-1; LIS3; Tuba-1; TUBA1; TUBA1A; TUBA3; TUBA6

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UniProt ID: (Human) Q71U36, (Mouse) Q5XJF8, (Rat) P68370

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Entrez Gene ID: (Chicken) 429035, (Human) 7846, (Mouse) 22142, (Rat) 64158

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Function(s)

GTPase activity structural constituent of cytoskeleton GTP binding structural molecule activity protein binding protein domain specific binding nucleotide binding protein heterodimerization activity tubulin

Process(es)

microtubule cytoskeleton organization mitotic cell cycle microtubule-based process G2/M transition of mitotic cell cycle cytoskeleton organization cytoskeleton-dependent intracellular transport cell division protein polymerization metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TUBA1A Polyclonal Antibody

TUBA1A Antibody (PA5-29444) in ICC/IF

Product Details

PA5-29444

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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