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Invitrogen
Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (002)
FIGURE: 1 / 1
Thioredoxin 1 Antibody (MA5-29839) in WB
Western Blot using Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (2) (Product # MA5-29839) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa.
Product Details
MA5-29839
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
002
Immunogen
Recombinant Mouse Thioredoxin/TXN/SASP Protein (Met1-Val105)
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785658
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Mouse Thioredoxin/TXN/SASP.
Target Information
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADF; ATL-derived factor; DKFZp686B1993; MGC61975; RP11-427L11.1; Thioredoxin; Trx
Gene Aliases: ADF; AW550880; Trx1; Txn; Txn1
UniProt ID: (Mouse) P10639
Entrez Gene ID: (Mouse) 22166
sulfate assimilation
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
protein folding
glycerol ether metabolic process
transport
response to radiation
positive regulation of peptidyl-serine phosphorylation
regulation of protein import into nucleus, translocation
cellular response to oxidative stress
positive regulation of DNA binding
cell redox homeostasis
negative regulation of protein export from nucleus
oxidation-reduction process
negative regulation of hydrogen peroxide-induced cell death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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