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Invitrogen
Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (JF101-8)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Thioredoxin 1 Antibody (MA5-32557) in IHC (P)
Immunohistochemistry analysis of Thioredoxin 1 with paraffin-embedded human small intestine. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal Thioredoxin 1 (Product # MA5-32557) at a dilution of 1:400 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted wit... View More
Product Details
MA5-32557
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JF101-8
Immunogen
Synthetic peptide within Human Thioredoxin aa 78-105
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809834
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADF; ATL-derived factor; DKFZp686B1993; MGC61975; RP11-427L11.1; SASP; Surface-associated sulphydryl protein; testicular tissue protein Li 199; Thioredoxin; thioredoxin delta 3; Trx; TXN delta 3
Gene Aliases: ADF; AW550880; TRDX; TRX; TRX1; TXN; Txn1
UniProt ID: (Human) P10599, (Rat) P11232, (Mouse) P10639
Entrez Gene ID: (Human) 7295, (Rat) 116484, (Mouse) 22166
sulfate assimilation
negative regulation of transcription from RNA polymerase II promoter
response to reactive oxygen species
transcription, DNA-templated
protein folding
glycerol ether metabolic process
movement of cell or subcellular component
signal transduction
cell-cell signaling
cell proliferation
response to radiation
nucleobase-containing small molecule interconversion
protein repair
activation of protein kinase B activity
positive regulation of peptidyl-serine phosphorylation
regulation of protein import into nucleus, translocation
positive regulation of DNA binding
cell redox homeostasis
negative regulation of protein export from nucleus
positive regulation of protein kinase B signaling
oxidation-reduction process
negative regulation of hydrogen peroxide-induced cell death
regulation of transcription, DNA-templated
response to selenium ion
response to activity
cellular response to drug
response to drug
response to axon injury
cellular response to antibiotic
cellular response to glucose stimulus
cellular response to hyperoxia
response to dexamethasone
response to thyroxine
transport
cellular response to oxidative stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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