Invitrogen

Thrombomodulin Monoclonal Antibody (141C01 (1009))

Thrombomodulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

Thrombomodulin Antibody (MA5-11454) in ICC/IF

Immunofluorescent analysis of CD141 (green) showing staining in the membrane of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD141 monoclonal antibody (Product # MA5-11454) in 3% BSA-PBS at a dilu... View More

Thrombomodulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Thrombomodulin Antibody in Flow Cytometry (Flow)

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Product Details

MA5-11454

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

Immunocytochemistry (ICC/IF)

1:10-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

1:2

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

141C01 (1009)

Immunogen

Recombinant protein encoding the six repeated EGF domains of human thrombomodulin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_10985845

Product Specific Information

MA5-11454 targets CD141 in IHC (P), FACS and ICC/IF applications and shows reactivity with Human and Rat samples.

The MA5-11454 immunogen is recombinant protein encoding the six repeated EGF domains of human thrombomodulin.

Target Information

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cellular and gene signatures of tumor-infiltrating dendritic cells and natural-killer cells predict prognosis of neuroblastoma.

Nature communications

Melaiu O,Chierici M,Lucarini V,Jurman G,Conti LA,De Vito R,Boldrini R,Cifaldi L,Castellano A,Furlanello C,Barnaba V,Locatelli F,Fruci D

MA5-11454 was used in Immunohistochemistry to show a key prognostic role of dendritic cells and natural killer cells in the prognosis of neuroblastoma.

Wed Nov 25 00:00:00 EST 2020

Low grade urothelial carcinoma mimicking basal cell hyperplasia and transitional metaplasia in needle prostate biopsy.

International braz j urol : official journal of the Brazilian Society of Urology

Arista-Nasr J,Martinez-Benitez B,Bornstein-Quevedo L,Aguilar-Ayala E,Aleman-Sanchez CN,Ortiz-Bautista R

MA5-11454 was used in immunohistochemistry to study low grade urothelial carcinoma mimicking transitional metaplasia and basal cell hyperplasia in needle prostate biopsy

Wed Mar 08 00:00:00 EST 2017

The endothelial microenvironment in the venous valvular sinus: thromboresistance trends and inter-individual variation.

Histochemistry and cell biology

Trotman WE,Taatjes DJ,Callas PW,Bovill EG

MA5-11454 was used in immunohistochemistry to examine the gene expression level in different regions of valve sinus

Tue Feb 01 00:00:00 EST 2011

Cellular regulation of blood coagulation: a model for venous stasis.

Blood

Campbell JE,Brummel-Ziedins KE,Butenas S,Mann KG

MA5-11454 was used in immunocytochemistry to develop a model system to study venous stasis.

Thu Dec 23 00:00:00 EST 2010

The urokinase receptor supports tumorigenesis of human malignant pleural mesothelioma cells.

American journal of respiratory cell and molecular biology

Tucker TA,Dean C,Komissarov AA,Koenig K,Mazar AP,Pendurthi U,Allen T,Idell S

MA5-11454 was used in immunohistochemistry to investigate the role of urokinase receptor in the pathogenesis of malignant pleural mesothelioma

Tue Jun 01 00:00:00 EDT 2010

Valves of the deep venous system: an overlooked risk factor.

Blood

Brooks EG,Trotman W,Wadsworth MP,Taatjes DJ,Evans MF,Ittleman FP,Callas PW,Esmon CT,Bovill EG

MA5-11454 was used in immunohistochemistry to examine the rolo of valvular sinus endothelium in thrombosis

Thu Aug 06 00:00:00 EDT 2009

Role of impaired peritubular capillary and hypoxia in progressive interstitial fibrosis after 56 subtotal nephrectomy of rats.

Nephrology (Carlton, Vic.)

Zhang B,Liang X,Shi W,Ye Z,He C,Hu X,Liu S

MA5-11454 was used in immunohistochemistry to study the potential pathogenic mechanisms underlying interstitial fibrosis after renal ablation in rats

Mon Aug 01 00:00:00 EDT 2005

Cell fusion-independent differentiation of neural stem cells to the endothelial lineage.

Nature

Wurmser AE,Nakashima K,Summers RG,Toni N,D'Amour KA,Lie DC,Gage FH

MA5-11454 was used in immunocytochemistry to investigate the stem-cell plasticity of mouse neural stem cells

Thu Jul 15 00:00:00 EDT 2004

Confocal laser scanning microscope study of cytokeratin immunofluorescence differences between villous and extravillous trophoblast: cytokeratin downregulation in pre-eclampsia.

Microscopy research and technique

Ockleford CD,Smith RK,Byrne S,Sanders R,Bosio P

MA5-11454 was used in immunohistochemistry to study differences in cytokeratin levels between villous and extravillous trophoblasts using confocal laser scanning microscopy

Sat May 01 00:00:00 EDT 2004

Healthy and pre-eclamptic placental basal plate lining cells: quantitative comparisons based on confocal laser scanning microscopy.

Microscopy research and technique

Smith RK,Ockleford CD,Byrne S,Bosio P,Sanders R

MA5-11454 was used in immunohistochemistry to study the immunocytochemical differences between placental basal plate lining cells from normal and pre-eclamptic placentae

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: BDCA-3; CD141; CD141 antigen; DC141; Fetomodulin; sCD141; soluble CD141; thrombomdulin; Thrombomodulin; TM

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Gene Aliases: AHUS6; BDCA3; CD141; THBD; THPH12; THRM; TM

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UniProt ID: (Human) P07204

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Entrez Gene ID: (Human) 7056, (Rat) 83580

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Function(s)

receptor activity transmembrane signaling receptor activity calcium ion binding protein binding molecular_function transmembrane signal receptor

Process(es)

signal transduction female pregnancy blood coagulation response to X-ray negative regulation of platelet activation negative regulation of blood coagulation response to lipopolysaccharide leukocyte migration response to cAMP negative regulation of fibrinolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Thrombomodulin Monoclonal Antibody (141C01 (1009))

Thrombomodulin Antibody (MA5-11454) in ICC/IF

Product Details

MA5-11454

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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