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Thrombopoietin Antibody (500-P49BT-25UG) in ELISA
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Thrombopoietin Polyclonal Antibody, Biotin (Product # 500-P49BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Thrombopoietin Polyclonal Antibody (Product # 500-P49-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO.
Product Details
500-P49BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 18.6 kDa Recombinant Human TPO
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930585
Product Specific Information
AA Sequence of recombinant protein: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TPO. Anti-Human TPO-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hTPO by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TPO (500-P49) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hTPO.
Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hTPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P49BT-1MG will be provided as 2 x 500 µg
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; ML; MPL ligand; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; Thrombopoietin
Gene Aliases: MGDF; MKCSF; ML; MPLLG; THCYT1; THPO; TPO
UniProt ID: (Human) P40225
Entrez Gene ID: (Human) 7066
positive regulation of protein phosphorylation
multicellular organism development
cell proliferation
myeloid cell differentiation
thrombopoietin-mediated signaling pathway
positive regulation of megakaryocyte differentiation
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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