Rockland

Thyroid Hormone Receptor beta 1 Polyclonal Antibody

View all (8) THRB antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

600-401-A96

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:1,000
-

ELISA (ELISA)

1:20,000-1:200,000
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-TR ß1 antibody is affinity purified from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminal of human THRB isoform 1 protein.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.15 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with human THRB protein. A BLAST analysis was used to suggest cross-reactivity with THRB from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRB from other sources has not been determined.

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR beta; TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta

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Gene Aliases: C-ERBA-2; C-ERBA-BETA; c-erbAbeta; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3R[b]; T3Rbeta; T3rec; THR1; THRB; THRB1; THRB2; TRbeta

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UniProt ID: (Human) P10828, (Mouse) P37242

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Entrez Gene ID: (Human) 7068, (Mouse) 21834, (Rat) 24831

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Function(s)
DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription corepressor activity thyroid hormone receptor activity protein binding zinc ion binding enzyme binding chromatin DNA binding sequence-specific DNA binding thyroid hormone binding double-stranded DNA binding protein homodimerization activity metal ion binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator
Process(es)
negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated transcription initiation from RNA polymerase II promoter sensory perception of sound negative regulation of female receptivity regulation of heart contraction female courtship behavior organ morphogenesis intracellular receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter Type I pneumocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation regulation of lipid metabolic process positive regulation of chondrocyte differentiation positive regulation of ossification negative regulation of transcription, DNA-templated regulation of cholesterol metabolic process regulation of triglyceride metabolic process embryonic organ development
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