Invitrogen

H3K9me3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Peptide array to ensure that the antibody binds to the antigen stated.

5 Published Figures

12 References

View all (17) H3K9me3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

H3K9me3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

H3K9me3 Antibody (49-1008) in ICC/IF

Immunofluorescence analysis of Tri-Methyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # 49-1008) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

H3K9me3 Antibody in Peptide array (Array)

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Product Details

49-1008

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:100-1:1,000

ChIP assay (ChIP)

View 6 publications 6 publications

Peptide Array (Array)

0.25 µg/mL

Dot blot (DB)

1:20,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Arthropod, Fish, Human, Mouse, Plant, Zebrafish

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

rabbits against histone H3, trimethylated at lysine 9 [K9me3], using a KLH-conjugated synthetic peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_2533859

Product Specific Information

49-1008, H3K9me3 antibody, has been shown to recognize plant species such as Arabidopsis and silena latifolia.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Misexpression of genes lacking CpG islands drives degenerative changes during aging.

Science advances

Lee JY,Davis I,Youth EHH,Kim J,Churchill G,Godwin J,Korstanje R,Beck S

49-1008 was used in Western Blot to report evidence that CGI- gene up-regulation is directly responsible for age-related physiological deterioration, notable for increased secretion of inflammation mediators.

Fri Dec 17 00:00:00 EST 2021

Misexpression of genes lacking CpG islands drives degenerative changes during aging.

Science advances

Lee JY,Davis I,Youth EHH,Kim J,Churchill G,Godwin J,Korstanje R,Beck S

Fri Dec 17 00:00:00 EST 2021

Natural Antisense Transcript PEBP1P3 Regulates the RNA Expression, DNA Methylation and Histone Modification of CD45 Gene.

Genes

Su Z,Liu G,Zhang B,Lin Z,Huang D

49-1008 was used in ChIP assay to investigate if the natural antisense RNA PEBP1P3 regulate the alternative splicing of CD45 RNA.

Mon May 17 00:00:00 EDT 2021

Inducing and exploiting vulnerabilities for the treatment of liver cancer.

Nature

Wang C,Vegna S,Jin H,Benedict B,Lieftink C,Ramirez C,de Oliveira RL,Morris B,Gadiot J,Wang W,du Chatinier A,Wang L,Gao D,Evers B,Jin G,Xue Z,Schepers A,Jochems F,Sanchez AM,Mainardi S,Te Riele H,Beijersbergen RL,Qin W,Akkari L,Bernards R

49-1008 was used in Immunocytochemistry to show that pharmacological inhibition of the DNA-replication kinase CDC7 induces senescence selectively in liver cancer cells with mutations in TP53.

Tue Oct 01 00:00:00 EDT 2019

Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.

The Journal of cell biology

Cuellar TL,Herzner AM,Zhang X,Goyal Y,Watanabe C,Friedman BA,Janakiraman V,Durinck S,Stinson J,Arnott D,Cheung TK,Chaudhuri S,Modrusan Z,Doerr JM,Classon M,Haley B

Published figure using Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # 49-1008) in ChIP assay

Mon Nov 06 00:00:00 EST 2017

DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop.

Experimental cell research

Sengupta D,Deb M,Rath SK,Kar S,Parbin S,Pradhan N,Patra SK

491008 was used in ChIP assay to clarify the link between miR-152 and CDH1 function

Mon Aug 15 00:00:00 EDT 2016

Epigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer.

Gene

Deb M,Sengupta D,Kar S,Rath SK,Roy S,Das G,Patra SK

49-1008 was used in ChIP assay to analyze epigenetic drift towards histone modifications and how they regulate CAV1 gene expression in colon cancer

Mon Apr 25 00:00:00 EDT 2016

Upregulating endogenous genes by an RNA-programmable artificial transactivator.

Nucleic acids research

Fimiani C,Goina E,Mallamaci A

49-1008 was used in immunocytochemistry to develop a novel, programmable transcription factor prototype.

Fri Sep 18 00:00:00 EDT 2015

p107-Dependent recruitment of SWI/SNF to the alkaline phosphatase promoter during osteoblast differentiation.

Bone

Flowers S,Patel PJ,Gleicher S,Amer K,Himelman E,Goel S,Moran E

Published figure using Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # 49-1008) in ChIP assay

Mon Dec 01 00:00:00 EST 2014

Long non-coding RNA urothelial carcinoma associated 1 induces cell replication by inhibiting BRG1 in 5637 cells.

Oncology reports

Wang X,Gong Y,Jin B,Wu C,Yang J,Wang L,Zhang Z,Mao Z

Published figure using Tri-Methyl-Histone H3 (Lys9) polyclonal antibody (Product # 49-1008) in Western Blot

Mon Sep 01 00:00:00 EDT 2014

FBXO22 protein is required for optimal synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-serine.

The Journal of biological chemistry

Dikopoltsev E,Foltyn VN,Zehl M,Jensen ON,Mori H,Radzishevsky I,Wolosker H

49-1008 was used in western blot to investigate the regulation of d-serine synthesis

Fri Dec 05 00:00:00 EST 2014

Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells.

Molecular endocrinology (Baltimore, Md.)

Kuroda K,Venkatakrishnan R,Salker MS,Lucas ES,Shaheen F,Kuroda M,Blanks A,Christian M,Quenby S,Brosens JJ

49-1008 was used in western blot to study the effects of glucocorticoids at the feto-maternal interface.

Fri Feb 01 00:00:00 EST 2013

Cross-species genomic and epigenomic landscape of retinoblastoma.

Oncotarget

Benavente CA,McEvoy JD,Finkelstein D,Wei L,Kang G,Wang YD,Neale G,Ragsdale S,Valentine V,Bahrami A,Temirov J,Pounds S,Zhang J,Dyer MA

49-1008 was used in ChIP assay to analyze the cross-species genomic and epigenomic pattern of retinoblastoma

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K9; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3-clustered histone 2; H3-clustered histone 3; H3-clustered histone 4; H3-clustered histone 6; H3-clustered histone 7; H3/b; H3/d; H3/f; H3/j; H3K9; H3Lys9me3; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; Histone H3.1; Histone H3.2; Histone H3.3; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K9me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K9; Tri-Methyl-H3 Lys9; Tri-Methyl-Histone H3 K9; Tri-Methyl-Histone H3 Lys9; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: H3; H3-143; H3-291; h3-5; H3-53; H3-614; H3-B; H3-F; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3a; H3b; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3F1K; H3F2; h3f3a; h3f3b.1; h3f3c; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; Hist1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; M32461; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731

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UniProt ID: (Human) P68431, (Human) Q71DI3, (Mouse) P68433, (Mouse) P84228, (Mouse) Q8CGN9, (Zebrafish) Q6PI20

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Entrez Gene ID: (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961, (Mouse) 360198, (Mouse) 319150, (Mouse) 260423, (Mouse) 319152, (Mouse) 97114, (Zebrafish) 550262

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Function(s)

DNA binding protein binding histone binding protein heterodimerization activity cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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H3K9me3 Polyclonal Antibody

H3K9me3 Antibody (49-1008) in ICC/IF

Product Details

49-1008

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

Peptide Array (Array)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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