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Invitrogen
Trop2 (EGP-1) Monoclonal Antibody (MR54), eBioscience™
FIGURE: 1 / 2
Trop2 (EGP-1) Antibody (14-6024-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human Trop2 (EGP-1) Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-6024-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
MR54
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10853488
Product Specific Information
Description: This MR54 monoclonal antibody reacts with human Trop-2, which is also known as Tumor-Associated Calcium Signal Transducer 2 (TACSTD2) and EGP-1. This type I transmembrane glycoprotein is expressed on trophoblast (syncytiotrophoblast and cytotrophoblast), epithelial, and most human carcinoma cells. Trop-2 plays a role in calcium-mediated signaling. Trop-2 has also been reported as an oncogene, with expression being correlated with tumorigenesis, metastasis, and anchorage-independent growth.
Applications Reported: This MR54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P), ELISA, and immunocytochemistry (ICC).
Applications Tested: This MR54 antibody has been tested by flow cytometry and immunocytochemistry on MCF7 cells as well as by immunohistochemistry of formalin-fixed paraffin embedded tissue using either low or high pH antigen retrieval methods. Use of high pH antigen retrieval results in better staining intensity. This can be used at less than of equal to 5 µg/mL. Is is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40kD glycoprotein, identified by monoclonal antibody GA733; Cell surface glycoprotein Trop-2; cell surface glycoprotein TROP2; epithelial glycoprotein-1; GA733; gastrointestinal tumor-associated antigen GA7331; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331; TACD2; truncated TACSTD2; Tumor-associated calcium signal transducer 2
Gene Aliases: EGP-1; EGP1; GA733-1; GA7331; GP50; M1S1; TACSTD2; TROP2
UniProt ID: (Human) P09758
Entrez Gene ID: (Human) 4070
cell surface receptor signaling pathway
visual perception
cell proliferation
negative regulation of epithelial cell migration
regulation of epithelial cell proliferation
negative regulation of stress fiber assembly
ureteric bud morphogenesis
negative regulation of branching involved in ureteric bud morphogenesis
cell-cell adhesion
negative regulation of substrate adhesion-dependent cell spreading
negative regulation of ruffle assembly
negative regulation of cell motility
positive regulation of stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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