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FIGURE: 1 / 2
TrxR1 Antibody (MA5-35564) in WB
Western blot analysis of TrxR1 in extracts of various cell lines. Samples were incubated with TrxR1 Monoclonal antibody (Product # MA5-35564) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-35564
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1106
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-649 of human Thioredoxin reductase 1 (TXNRD1 ) (Q16881)
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849464
Product Specific Information
Immunogen sequence: MGCAEGKAVA AAAPTELQTK GKNGDGRRRS AKDHHPGKTL PENPAGFTST ATADSRALLQ AYIDGHSVVI FSRSTCTRCT EVKKLFKSLC VPYFVLELDQ TEDGRALEGT LSELAAETDL PVVFVKQRKI GGHGPTLKAY QEGRLQKLLK MNGPEDLPKS YDYDLIIIGG GSGGLAAAKE AAQYGKKVMV LDFVTPTPLG TRWGLGGTCV NVGCIPKKLM HQAALLGQAL QDSRNYGWKV EETVKHDWDR MIEAVQNHIG SLNWGYRVAL REKKVVYENA YGQFIGPHRI KATNNKGKEK IYSAERFLIA TGERPRYLGI PGDKEYCISS DDLFSLPYCP GKTLVVGASY VALECAGFLA GIGLDVTVMV RSILLRGFDQ DMANKIGEHM EEHGIKFIRQ FVPIKVEQIE AGTPGRLRVV AQSTNSEEII EGEYNTVMLA IGRDACTRKI GLETVGVKIN EKTGKIPVTD EEQTNVPYIY AIGDILEDKV ELTPVAIQAG RLLAQRLYAG STVKCDYENV PTTVFTPLEY GACGLSEEKA VEKFGEENIE VYHSYFWPLE WTIPSRDNNK CYAKIICNTK DNERVVGFHV LGPNAGEVTQ GFAAALKCGL TKKQLDSTIG IHPVCAEVFT TLSVTKRSGA SILQAGCUG
Target Information
This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; MGC9145; NADPH-dependent thioredoxin reductase; oxidoreductase; Peroxidase TXNRD1; selenoprotein oxidoreductase; testis tissue sperm-binding protein Li 46a; Thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; TR alpha
Gene Aliases: GRIM-12; GRIM12; KDRF; TR; TR1; TRXR1; TXNR; TXNRD1
UniProt ID: (Human) Q16881, (Mouse) Q9JMH6
Entrez Gene ID: (Human) 7296, (Rat) 58819, (Mouse) 50493
thioredoxin-disulfide reductase activity
protein binding
electron carrier activity
protein disulfide oxidoreductase activity
flavin adenine dinucleotide binding
methylselenol reductase activity
methylseleninic acid reductase activity
mercury (II) reductase activity
NAD(P)H oxidase activity
selenate reductase activity
protein homodimerization activity
mercury ion binding
NADP binding
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
reductase
response to reactive oxygen species
mesoderm formation
selenium compound metabolic process
signal transduction
cell proliferation
nucleobase-containing small molecule interconversion
cell redox homeostasis
oxidation-reduction process
cellular oxidant detoxification
placenta development
glutathione metabolic process
response to oxidative stress
gastrulation
response to selenium ion
positive regulation of cell death
selenocysteine metabolic process
methylmercury metabolic process
response to drug
benzene-containing compound metabolic process
hydrogen peroxide catabolic process
response to axon injury
detoxification of mercury ion
protein tetramerization
response to hyperoxia
halogen metabolic process
NADPH oxidation
cellular response to copper ion
cellular response to hyperoxia
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