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Trypsin Antibody (200-401-139-0100) in WB
Western Blot of Rabbit anti-Trypsin antibody. Lane 1: Trypsin (Bovine). Load: 50 ng per lane. Primary antibody: Trypsin antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 25 kDa for Trypsin. Other band(s): Low molecular weight bands could correspond to autocatalytic fragments.
Product Details
200-401-139-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin (Bovine Pancreas). Cross-reactivity against Trypsin from other tissues and species may occur but has not been specifically determined.
Target Information
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anionic trypsin I; Anionic trypsin-I; Beta-trypsin; Cationic trypsin; MGC120175; MGC149362; Pretrypsinogen I; Protease Serine 1; Serine protease 1; TRYP8; Trypsin I; Trypsin-I; trypsinogen
Gene Aliases: PRSS1; TRP1; TRY1; TRYP1
UniProt ID: (Bovine) P00760
Entrez Gene ID: (Bovine) 615026
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