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Trypsin Antibody (PA5-145237) in WB
Western blot analysis of Trypsin in various lysates. Samples were incubated with Trypsin polyclonal antibody (Product # PA5-145237) using a dilution of 0.2 µg/mL followed by HRP-Linked Caprine Anti-Rabbit IgG secondary antibody at a dilution of 0.2 µg/mL. Lane 1: Porcine Pancreas lysate; Lane 2: Rat Pancreas lysate.
Product Details
PA5-145237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Pig TRY protein, Ile9-Asn231 (Accession #P00761)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3093555
Target Information
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anionic trypsin I; Anionic trypsin-1; Anionic trypsin-I; Beta-trypsin; Cationic trypsinogen; digestive zymogen; MGC120175; MGC149362; nonfunctional trypsin 1; pancreatic trypsin 1; Pretrypsinogen I; Protease Serine 1; protease, serine, 2; Serine protease 1; TCR V beta 4.1; TRYP8; Trypsin I; Trypsin-1; trypsinogen; trypsinogen 1; trypsinogen A
Gene Aliases: PRSS1; PTRYI; RATPTRYI; TRP1; TRY1; TRY4; TRYP1
UniProt ID: (Human) P07477, (Rat) P00762
Entrez Gene ID: (Human) 5644, (Rat) 24691
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