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Trypsin Inhibitor Antibody (100-4179) in WB
Rockland Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 µg of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Rockland Rabbit anti Soybean Trypsin Inhibitor antibody (200-4679 lot 6594 1:5000 in MB-0070, ON 4 C). Primary antibody was detected with Rockland Dylight 488 c... View More
Product Details
100-4179
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Trypsin Inhibitor [Soy Bean]
Conjugate
Form
Lyophilized
Concentration
85 mg/mL
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
Target Information
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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