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TurboLuc luciferase Antibody (PA1-178) in WB
Western blot analysis of TurboLuc luciferase was performed by loading the indicated amounts of recombinant TurboLuc whole cell lysates, and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for 1 hour at room temperature. TurboLuc luciferase was detected at 16 kDa using a TurboLuc luciferase polyclonal antibody (Produc... View More
Product Details
PA1-178
Product Specifications
Species Reactivity
Luciferase
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified recombinant TurboLuc luciferase expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539909
Product Specific Information
Western blot analysis of PA1-178 detects an ~16-18 kDa protein in Tluc expressing cells.
PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.
Target Information
TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family. The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression. Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light. Tluc is smaller and brighter than other commonly used luciferases, including firefly and Renilla. The intense bioluminescence greatly enhances sensitivity of Tluc luciferase-based assays, enabling detection of very minute amounts of luciferase (signal) in very sensitive assays such as protein interaction qIP assays (Prod # 82032, 82033, 82035, 82036).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: TurboLuc luciferase synthetic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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