Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
U2AF2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
U2AF2 Antibody (PA5-30442) in ICC/IF
Immunofluorescence analysis of U2AF2 was performed using 70% confluent A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with U2AF2 Polyclonal Antibody (Product # PA5-30442) at 5µg/mL concentration in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a ... View More
Product Details
PA5-30442
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 252 and 426 of Human U2AF65
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547916
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji.
Predicted reactivity: Mouse (100%), Xenopus laevis (96%), Pig (100%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hU2AF(65); hU2AF65; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), 65 kDa; U2 snRNP auxiliary factor large subunit
Gene Aliases: 65kDa; U2AF2; U2AF65
UniProt ID: (Human) P26368, (Mouse) P26369
Entrez Gene ID: (Human) 11338, (Mouse) 22185
mRNA splicing, via spliceosome
termination of RNA polymerase II transcription
mRNA processing
RNA export from nucleus
mRNA export from nucleus
mRNA 3'-end processing
positive regulation of RNA splicing
negative regulation of mRNA splicing, via spliceosome
regulation of mitophagy
positive regulation of protein targeting to mitochondrion
RNA splicing
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support