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UBA5 Antibody (A304-114A) in IP
Detection of human UBA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-UBA5 antibody A304-114A (lot A304-114A-1) used for IP at 6 µg/mg lysate. UBA5 was also immunoprecipitated by rabbit anti-UBA5 antibody A304-115A. For blotting immunoprecipitated UBA5, A304-115A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A304-114A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 125 to 175 of Human Ubiquitin-Like Modifier Activating Enzyme 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Chicken, Bovine and Orangutan
Target Information
This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ23251; FLJ23251UBA5; ThiFP1; UBA5, ubiquitin-activating enzyme E1 homolog; Ubiquitin-activating enzyme 5; Ubiquitin-activating enzyme E1 domain-containing protein 1; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-like modifier activating enzyme 5; Ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme
Gene Aliases: THIFP1; UBA5; UBE1DC1
UniProt ID: (Human) Q9GZZ9
Entrez Gene ID: (Human) 79876
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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