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Product Details
MA5-34786
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JG38-68
Immunogen
Recombinant protein within Human Ube2N aa 1-93
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
RRID
AB_2848694
Product Specific Information
Positive Control: Daudi, SH-SY-5Y, mouse spleen tissue lysate, rat spleen tissue lysate, human colon tissue, human prostate cancer tissue, mouse testis tissue.
Target Information
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. (Uniprot)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; MGC131857; MGC8489; Ubc13; UbcH13; Ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog
Gene Aliases: BLU; HEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN; UBC13; UBE2N
UniProt ID: (Human) P61088
Entrez Gene ID: (Human) 7334
double-strand break repair via homologous recombination
DNA double-strand break processing
stimulatory C-type lectin receptor signaling pathway
regulation of DNA repair
postreplication repair
double-strand break repair via nonhomologous end joining
cellular protein modification process
proteolysis
ubiquitin-dependent protein catabolic process
protein ubiquitination
histone ubiquitination
positive regulation of histone modification
regulation of histone ubiquitination
Fc-epsilon receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of DNA repair
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of ubiquitin-protein transferase activity
nucleotide-binding oligomerization domain containing signaling pathway
protein K63-linked ubiquitination
global genome nucleotide-excision repair
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