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UFL1 Antibody (A303-455A) in IP
Detection of human ULF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ULF1 antibody A303-455A used for IP at 6 µg/mg lysate. ULF1 was also immunoprecipitated by rabbit anti-ULF1 antibody A303-456A, which recognizes a downstream epitope. For blotting immunoprecipitated ULF1, A303-456A was used at 0.4 µg/ml,. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A303-455A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 175 and 225 of Human E3 UFM1-Protein Ligase 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
UFL1 (UFM1 Specific Ligase 1) is a Protein Coding gene. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System. Gene Ontology (GO) annotations related to this gene include ligase activity and UFM1 transferase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 UFM1-protein ligase 1; E3 UFM1-protein transferase 1; LZAP-binding protein; Multiple alpha-helix protein located at ER; Novel LZAP-binding protein; Regulator of C53/LZAP and DDRGK1; Regulator of CDK5RAP3 and DDRGK1; RP3-393D12.1; UFM1-specific ligase 1
Gene Aliases: KIAA0776; MAXER; NLBP; RCAD; UFL1
UniProt ID: (Human) O94874
Entrez Gene ID: (Human) 23376
osteoblast differentiation
positive regulation of cell proliferation
negative regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of protein localization
regulation of intracellular estrogen receptor signaling pathway
response to endoplasmic reticulum stress
positive regulation of glial cell proliferation
protein ufmylation
response to L-glutamate
protein K69-linked ufmylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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