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UHRF1 Antibody (PA5-49428) in WB
Western blot analysis of UHRF1 in mouse thymus lysates. Samples were incubated with UHRF1 polyclonal antibody (Product # PA5-49428) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size: 88 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-49428
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 596-628 amino acids from the C-terminal region of Mouse Uhrf1.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2634882
Target Information
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; MGC138707; mUhrf1; Nuclear protein 95; Nuclear zinc finger protein Np95; RING-type E3 ubiquitin transferase UHRF1; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Aliases: AL022808; ICBP90; Np95; RNF106; Uhrf1
UniProt ID: (Mouse) Q8VDF2
Entrez Gene ID: (Mouse) 18140
core promoter proximal region sequence-specific DNA binding
DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
methyl-CpG binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
nucleosomal histone binding
methylated histone binding
histone binding
identical protein binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
hemi-methylated DNA-binding
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
cell cycle
cell proliferation
maintenance of DNA methylation
histone monoubiquitination
chromatin modification
histone ubiquitination
positive regulation of cellular protein metabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein autoubiquitination
regulation of methylation-dependent chromatin silencing
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