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Invitrogen
UHRF1 Recombinant Superclonal Antibody (1HCLC)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
UHRF1 Antibody (711865) in WB
Western blot analysis was performed on Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1) Hep G2 (Lane 2), HCT 116 (Lane 3), COLO 205 (Lane 4), Jurkat (Lane 5), THP-1 (Lane 6), MCF-7 (Lane 7), MDA-MB-231 (Lane 8), U-2 OS (Lane 9) and PANC-1 (Lane 10). The blots were probed with Anti-UHRF1 Recombinant Rabbit Polyclonal Antibody (Product # 711865, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilutio... View More
Product Details
711865
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
1HCLC
Immunogen
Protein corresponding to human UHRF1 (aa126-aa285)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2725628
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Rat.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ac2-121; E3 ubiquitin-protein ligase UHRF1; FLJ21925; Hnp95 Huhrf1; hUHRF1; HuNp95; Inverted CCAAT box-binding protein of 90 kDa; MGC138707; nuclear phosphoprotein 95; Nuclear protein 95; Nuclear zinc finger protein Np95; RING finger protein 106; RING-type E3 ubiquitin transferase UHRF1; Transcription factor ICBP90; ub; Ubiquitin-like PHD and RING finger domain-containing protein 1; ubiquitin-like with PHD and ring finger domains 1; Ubiquitin-like-containing PHD and RING finger domains protein 1
Gene Aliases: hNP95; hUHRF1; huNp95; ICBP90; NP95; RNF106; TDRD22; UHRF1
UniProt ID: (Human) Q96T88
Entrez Gene ID: (Human) 29128
core promoter proximal region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
methyl-CpG binding
ligase activity
nucleosomal histone binding
methylated histone binding
histone binding
identical protein binding
hemi-methylated DNA-binding
ubiquitin protein ligase activity
ubiquitin-protein ligase
negative regulation of transcription from RNA polymerase II promoter
DNA repair
transcription, DNA-templated
cell cycle
cell proliferation
maintenance of DNA methylation
histone monoubiquitination
histone ubiquitination
positive regulation of cellular protein metabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
protein autoubiquitination
regulation of methylation-dependent chromatin silencing
positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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